Continuous screening system for inhibited enzyme catalysis: A membrane reactor approach

Evgenij Lyagin (1) , Anja Drews (2) , Subhamoy Bhattacharya (3) , Marion Bettina Ansorge-Schumacher (4) , Matthias Kraume (1)

1 Chair of Chemical and Process Engineering
2 Dept. of Engineering II
3 Chair of Chemical and Process Engineering/Dept. of Enzyme Technology
4 Dept. of Enzyme Technology

The screening of catalysts, substrates or conditions in the early stages of bioprocess development requires an enormous number of experiments and is a tedious, expensive and time consuming task. Currently available screening systems can only be operated in batch or fed-batch mode which can lead to severe misinterpretations of screening results. For example, catalysts that are inhibited by substrates or accumulating products will be excluded from further investigations in the early stages of process development despite the fact that they might be superior to other candidates in a different operational mode. Important and advantageous properties such as turnover stability can also be overshadowed by product inhibition. The aim of this project thus is to develop a novel screening system which enables continuous feeding of substrates and continuous removal of products. A prototype based on the membrane reactor concept was designed and operated for the hydrolysis of cellulose as a model reaction for this feasibility study.

Mots clés: Life Sciences

Evgenij Lyagin, Anja Drews, Subhamoy Bhattacharya, Marion Bettina Ansorge-Schumacher, Matthias Kraume. Continuous screening system for inhibited enzyme catalysis: A membrane reactor approach. Biotechnology Journal, 2010, 5 (8), pp.813. ⟨10.1002/biot.201000130⟩. ⟨hal-00560296⟩