The Visualisation of Vitreous Using Surface Modified Poly(DL-lactic-co-glycolic acid) Microparticles
Résumé
Aims: To demonstrate in vitro the potential use of poly(lactic-co-glycolic acid) (PLGA) microparticles to aid visualisation of vitreous during anterior vitrectomy following posterior capsule rupture and to compare with the use of triamcinolone suspension. Methods: PLGA microparticles (diameter 10-60 µm) were fabricated using single and/or double emulsion technique(s) and used as untreated or following the surface adsorption of a protein (transglutaminase). Particle size, shape, morphology and surface topography were assessed using scanning electron microscopy (SEM) and calibrated against a standard triamcinolone suspension. Furthermore, the efficacy of these microparticles to enhance visualisation of vitreous against the triamcinolone suspension was assessed using an in vitro set-up exploiting porcine vitreous. Results: Unmodified PLGA microparticles displayed a degree of hydrophobic behaviour when in contact with porcine vitreous and also a high rate of dispersion following repeated wash cycles. In contrast, surface modified transglutaminase- PLGA microparticles demonstrated a significant improvement in its vitreophilic properties and were comparable to a triamcinolone suspension in its ability to enhance the visualisation of vitreous. Furthermore, this adhesive behaviour also demonstrated selectivity by not binding to the corneal endothelium. Conclusion: The use of transglutaminase-modified biodegradable PLGA microparticles may represent a novel method of visualising vitreous and aiding anterior vitrectomy. This method may provide a distinct alternative for the visualisation of vitreous whilst eliminating the pharmacological effects of triamcinolone acetonide suspension. Furthermore, the incorporation of drugs, growth factors and/or antibiotics within these microparticles may also be considered and exploited in many surgical applications.
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