Impact of plant extracts tested in attention-deficit/hyperactivity disorder treatment on cell survival and energy metabolism in human neuroblastoma SH-SY5Y cells
Résumé
Plant extracts like Hypericum perforatum and Pycnogenol® have been tested as alternatives to the classical ADHD drugs. It has been possible to describe neuroprotective effects of such plant extracts. A reduction of ADHD symptoms could be shown in clinical studies after the application of Pycnogenol®, which is a pine bark extract. The impacts of the standardized herbal extracts Hypericum perforatum, Pycnogenol® and Enzogenol® up to a concentration of 5.000 ng/ml on cell survival and energy metabolism in human SH-SY5Y neuroblastoma cells has been investigated in the present examination. Hypericum perforatum significantly decreased survival of cells after treatment with a concentration of 5000 ng/ml, whereas lower concentrations exerted no significant effects. Pycnogenol® induced a significant increase of cell survival after incubation with a concentration of 32.25 ng/ml and a concentration of 250 ng/ml. Other applied concentrations of Pycnogenol® failed to exert significant effects. Treatment with Enzogenol® did not lead to significant changes in cell survival. Concerning energy metabolism, treatment of cells with a concentration of 5000 ng/ml Hypericum perforatum led to a significant increase of ATP levels, whereas treatment with a concentration of 500 ng/ml had no significant effect. Incubation of cells with Pycnogenol® and Enzogenol® exerted no significant effects. None of the tested substances caused any cytotoxic effect when used in therapeutically-relevant concentrations.
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