PHYLOGENY AND RESISTANCE PROFILES OF HIV-1 POL SEQUENCES FROM RECTAL BIOPSIES AND BLOOD

Terese Lea Katzenstein (1) , Ann Berith Petersen (2) , Merete Storgaard (3) , Niels Obel (1) , Søren Jensen Fangel (3) , Claus Nielsen (2) , Louise Bruun Jørgensen (4)

1 Department of Infectious Diseases [Rigshospitalet]
2 Virology
3 Department of Infectious Diseases
4 Virology

The phylogeny and resistance profiles of human immunodeficiency virus type 1 (HIV-1) protease (PR) and reverse transcriptase (RT) sequences were compared among six patients with HIV-1 that had received numerous treatments. RNA and DNA fractions were obtained from concurrent blood and rectal biopsy samples. Phylogenetic trees and resistance profiles showed that the rectal mucosa and the peripheral blood mononuclear cells (PBMCs) harboured different HIV-1 strains. The resistance-associated mutations found in each strain corresponded to the treatment history of the patients. These resistance mutations acquired during earlier treatment regimens were detected in the sequences obtained from the rectal samples and in the PBMCs in several of the patients. Also, differences in the resistance profiles were observed between anatomical sites and between RNA and DNA fractions. Thus, a single sample probably will not be representative of the HIV-1 archived in different sites. Both the resistance profile and phylogeny of HIV-1 often differed in sequences obtained from RNA and DNA from the same site. These findings suggest that additional information regarding the antiviral resistance profile of the patient might be obtained by testing different anatomical sites.

Mots clés: Medicine

Terese Lea Katzenstein, Ann Berith Petersen, Merete Storgaard, Niels Obel, Søren Jensen Fangel, et al.. PHYLOGENY AND RESISTANCE PROFILES OF HIV-1 POL SEQUENCES FROM RECTAL BIOPSIES AND BLOOD. Journal of Medical Virology, 2010, 82 (7), pp.1103. ⟨10.1002/jmv.21796⟩. ⟨hal-00552409⟩