The PKC-ζ interacting protein ZIP3 is generated by intronic polyadenylation and is expressed in brain and retina of the rat
Résumé
Scaffold proteins contain multiple protein-protein interaction modules that physically assemble functional related proteins into larger complexes. Protein kinase C-ζ interacting proteins (ZIP) link the enzymatic activity of the atypical protein kinase C isoforms PKC-λ/ι or PKC-ζ to target proteins and are associated with neurodegenerative disorders. In the rat, alternative splicing generates 3 ZIP variants. Previously, we identified the ZIP3 transcript containing 13 C-terminal amino acids encoded by intron 4 in the rat central nervous system (CNS). Here, we identified intronic polyadenylation signals in rat and human ZIP genes and detected the corresponding ZIP3-like transcripts. In addition, we generated ZIP3 specific immunesera and observed expression of the protein in brain and retina of the adult rat. In the retina, ZIP3 is present in nuclear layers where it co-localizes with PKC-ζ. An immuneserum recognizing all 3 ZIP isoforms labelled the same cells as the newly generated ZIP3 specific antibodies and in addition stained both synaptic layers of the retina. There, ZIP proteins are localized in axon terminals of rod bipolar cells that also contain ZIP-interacting PKC-ζ and GABAC receptors. In summary, we detected ZIP3-like transcripts in rat and human and describe the expression of ZIP3 in the rat CNS.
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