Study of the transport of thyroid hormone by transporters of the SLC10 family

W. Edward Visser (1) , Wing S. Wong (1) , Alies A.A. van Mullem (1) , Edith C.H. Friesema (1) , Joachim Geyer (2) , Theo J. Visser (1)

1 Department of Internal Medicine
2 JLU - Justus-Liebig-Universität Gießen = Justus Liebig University

Transport of (sulfated) iodothyronines across the plasma membrane is required for their intracellular metabolism. Rat Na/taurocholate cotransporting polypeptide (Ntcp; Slc10a1) has been identified as an important transporter protein. We demonstrate that among the 7 members of the solute carrier family SLC10, only human SLC10A1 mediates sodium-dependent transport of the iodothyronine T4 and iodothyronine sulfates T3S and T4S. In contrast to SLC10A2-7, cells co-expressing SLC10A1 and the deiodinase D1 demonstrate a dramatic increase in T3S and T4S metabolism. The SLC10A1 substrates taurocholate, DHEAS and E3S inhibit T3S and T4S transport. Furthermore, co-transfection of SLC10A1 with CRYM, a well-known intracellular iodothyronine-binding protein, results in an enhanced intracellular accumulation of T3S and T4S, indicating that CRYM binds iodothyronine sulfates.

Mots clés: SLC10 family; SLC10A1; NTCP; transporter; thyroid hormone; iodothyronine (sulfates); taurocholate; CRYM; mu-crystallin

W. Edward Visser, Wing S. Wong, Alies A.A. van Mullem, Edith C.H. Friesema, Joachim Geyer, et al.. Study of the transport of thyroid hormone by transporters of the SLC10 family. Molecular and Cellular Endocrinology, 2009, 315 (1-2), pp.138. ⟨10.1016/j.mce.2009.08.003⟩. ⟨hal-00547654⟩