 |
|
|
|
| HAL : hal-00546528, version 1 |
| DOI : 10.1364/OE.18.026469 |
| Fiche détaillée |  Récupérer au format |
|
|
| Optics Express 18, 25 (2010) 26469 |
|
|
|
|
| Simultaneous calibration of optical tweezers spring constant and position detector response |
|
|
| Antoine Le Gall 1Karen Perronet 1 |
|
|
| (02/12/2010) |
|
|
|
| We demonstrate a fast and direct calibration method for systems using a single laser for optical tweezers and particle position detection. The method takes direct advantage of back-focal-plane interferometry measuring not an absolute but a differential position, i.e. the position of the trapped particle relative to the center of the optical tweezers. Therefore, a fast step-wise motion of the optical tweezers yields the impulse response of the trapped particle. Calibration parameters such as the detector's spatial and temporal response and the spring constant of the optical tweezers then follow readily from fitting the measured impulse response. |
|
|
|
|
|
|
|
|
|
|
|
| 1 : | Laboratoire Charles Fabry de l'Institut d'Optique (LCFIO) |
|
|
|
Institut d'Optique Graduate School (IOGS) – CNRS : UMR8501 – Université Paris XI - Paris Sud |
|
|
| 2 : | Departments of Physics, Molecular and Cellular Biology, and College of Optical Sciences, University of Arizona, Tucson |
|
|
|
Departments of Physics, Molecular and Cellular Biology, and College of Optical Sciences |
|
|
|
|
|
|
|
|
|
| lcf-oa, lcf-oa,bio |
|
|
|
|
| Domaine | : | Sciences du Vivant/Biochimie, Biologie Moléculaire/Biophysique |
|
|
| biophotonique |
|
|
|
| Liste des fichiers attachés à ce document : | |||||
|
|||||
|
|
|
| hal-00546528, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00546528 | |
| oai:hal.archives-ouvertes.fr:hal-00546528 | |
| Contributeur : Karen Perronet | |
| Soumis le : Mardi 14 Décembre 2010, 13:06:39 | |
| Dernière modification le : Vendredi 24 Février 2012, 14:12:59 | |
