Generation of avian influenza virus (AIV) contaminated fecal fine particulate matter (PM): genome and infectivity detection and calculation of immission

N. Sedlmaier , K. Hoppenheidt , H. Krist , S. Lehmann , H. Lang , M. Büttner

As a model for aerosol transmission, chicken feces was spiked with avian influenza virus (AIV) subtype H10N7 and used to generate a fine particulate matter aerosol. For this an innovative aerosol chamber was developed, that collected PM on quartz microfiber filters. With AIV contaminated PM-dust coated filters different incubation times ranging from 0 to 4 days and storage mainly at +4 and +20°C and at different relative humidity (RH) were performed. Embryonic death in inoculated hen's eggs with filter elute was the AIV infectivity read out. To determine viral genome presence quantitative real time RT-PCR was applied.

Mots clés: Avian influenza virus; Chicken feces; Fine particulate matter (PM); Airborne transmission; Quartz microfiber filters

N. Sedlmaier, K. Hoppenheidt, H. Krist, S. Lehmann, H. Lang, et al.. Generation of avian influenza virus (AIV) contaminated fecal fine particulate matter (PM): genome and infectivity detection and calculation of immission. Veterinary Microbiology, 2009, 139 (1-2), pp.156. ⟨10.1016/j.vetmic.2009.05.005⟩. ⟨hal-00520661⟩