HAL : hal-00515989, version 1

PubMed : 19603845

DOI : 10.1021/jp902540k

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The Journal of Physical Chemistry B 113, 29 (2009) 10008-13

Damage induced to DNA by low-energy (0-30 eV) electrons under vacuum and atmospheric conditions.

Emilie Brun 1, Pierre Cloutier 2, Cécile Sicard-Roselli 3, Michel Fromm 4, Léon Sanche 2

(23/07/2009)

In this study, we show that it is possible to obtain data on DNA damage induced by low-energy (0-30 eV) electrons under atmospheric conditions. Five monolayer films of plasmid DNA (3197 base pairs) deposited on glass and gold substrates are irradiated with 1.5 keV X-rays in ultrahigh vacuum and under atmospheric conditions. The total damage is analyzed by agarose gel electrophoresis. The damage produced on the glass substrate is attributed to energy absorption from X-rays, whereas that produced on the gold substrate arises from energy absorption from both the X-ray beam and secondary electrons emitted from the gold surface. By analysis of the energy of these secondary electrons, 96% are found to have energies below 30 eV with a distribution peaking at 1.4 eV. The differences in damage yields recorded with the gold and glass substrates is therefore essentially attributed to the interaction of low-energy electrons with DNA under vacuum and hydrated conditions. From these results, the G values for low-energy electrons are determined to be four and six strand breaks per 100 eV, respectively.

1 :	Laboratoire de Génie Electrique de Grenoble (G2ELab)
	CNRS : UMR5269 – Université Joseph Fourier - Grenoble I – Institut Polytechnique de Grenoble
2 :	Centre for Research in Radiooncology
	University de Sherbrooke (Canada)
3 :	umr 8000 (LCP)
	Université Paris XI - Paris Sud
4 :	Laboratoire de Chimie Physique et Rayonnements A. Chambaudet (UMR CEA E4)
	Université de Franche-Comté

Domaine

:

Chimie/Chimie théorique et/ou physique

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Contributeur : Michel Fromm <>

Soumis le : Mercredi 8 Septembre 2010, 14:35:26

Dernière modification le : Mercredi 8 Septembre 2010, 15:30:07