Caprine herpesvirus-1-specific IgG subclasses in naturally and experimentally infected goats

Mariarosaria Marinaro , Anna Lucia Bellacicco , Michele Camero , Elvira Tarsitano , Maria Tempesta , Antonio Cassone , Canio Buonavoglia

Enzyme-linked immunosorbent assays (ELISAs) were developed to detect caprine herpervirus-1 (CpHV-1)-specific IgG1 and IgG2 in sera from 43 naturally-infected goats. The analysis of the IgG subclasses showed a dual pattern of distribution in seropositive goats with a major group of animals (36 out of 43) exhibiting significantly higher levels of IgG2 over IgG1 and a minor group (7 out of 43) possessing equal levels of IgG1 and IgG2. Four goats were experimentally infected with a virulent CpHV-1 Ba.1 strain by the intranasal or the intravaginal route and the kinetics of appearance of CpHV-1-specific IgG, IgG1 and IgG2 in the serum were studied. Two weeks following infection, both IgG1 and IgG2 levels increased although convalescent sera (i.e., collected five to eight weeks post-infection) showed a clear prevalence of the IgG2 subclass. To determine the contribution of the different IgG subclasses to herpesvirus immunity, serum neutralization (SN) assays were performed in both naturally and experimentally infected goats. The kinetics of SN showed that neutralization activity was mainly associated to the IgG1 subclass and this was also confirmed in naturally infected goats. The results are discussed from the standpoint that the profile of the IgG subclasses is instrumental to study immune responses to CpHV-1 and that vaccination strategies may benefit from this information.

Mots clés: CpHV-1; IgG subclass; Viral neutralization; IgG; Goat immunity

Mariarosaria Marinaro, Anna Lucia Bellacicco, Michele Camero, Elvira Tarsitano, Maria Tempesta, et al.. Caprine herpesvirus-1-specific IgG subclasses in naturally and experimentally infected goats. Veterinary Microbiology, 2009, 138 (3-4), pp.266. ⟨10.1016/j.vetmic.2009.04.008⟩. ⟨hal-00514609⟩