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Article Dans Une Revue European Respiratory Journal Année : 2011

Hypersensitivity pneumonitis and metalworking fluids contaminated by Mycobacteria.

Résumé

Metalworking fluids are responsible for hypersensitivity pneumonitis (MWF-HP). The aim is to identify the antigen (Ag) responsible for MWF-HP and to optimize serological diagnosis by definition of a threshold allowing discrimination between HP patients from asymptomatic exposed workers (H-MWF) Thirteen patients who were workers at a car-engine manufacturing plant, were suspected of MWF-HP. Microbial analysis of 83 used MWF was carried out. Sera from 13 MWF-HP, 12 H-MWF and 18 UC (healthy unexposed controls) were tested to determine their immunological responses to three Ag including Mycobacterium immunogenum (Mi). Mi was identified in 40% of used fluids by culture and confirmed by DNA sequence. The threshold to differentiate MWF-HP from H-MWF was five arcs of precipitation (Sensitivity (Se) 77%, specificity (Sp) 92%) as determined by electrosyneresis. Using ELISA methods with protein extract from Mi, a threshold leading to (Se) 92% and (Sp) 100%) was established. The detection of specific antibodies against Mi Ag in high level in case sera, suggests that Mi contaminated-MWF is responsible for MWF-HP. To discriminate MWF-HP from H-MWF, we also put a five arcs threshold forward by electrosyneresis and a 1.6 arbitrary unit for ELISA methods threshold.

Domaines

Mycologie

Dates et versions

hal-00514090 , version 1 (01-09-2010)

Identifiants

Citer

I. Tillie-Leblond, F. Grenouillet, G. Reboux, S. Roussel, B. Chouraki, et al.. Hypersensitivity pneumonitis and metalworking fluids contaminated by Mycobacteria.. European Respiratory Journal, 2011, 37 (3), pp.640-7. ⟨10.1183/09031936.00195009⟩. ⟨hal-00514090⟩
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