The use of Self Interaction Chromatography in stable formulation and crystallization of proteins

Kedar Deshpande (1) , Tangir Ahamed (1) , Joop H. ter Horst (2) , Peter T. Jansens (2) , Luuk A.M. van Der Wielen (1) , Marcel Ottens (3)

1 Department of Biotechnology [Delft]
2 Process & Energy Laboratory
3 Biotechnology

This paper reviews the basic principles of the recently developed self-interaction chromatographic (SIC) technique in regard to protein solution stability and protein crystallization. It gives experimental protocols for both normal scale and micro scale SIC experiments, and reviews recent developments and current application of this novel technique in the biopharmaceutical area. This paper aims to be a benchmark in the further proliferation of this highly effective and fast technology for the rational design of stable aqueous formulations of therapeutic proteins and the determination of solution conditions favoring protein crystallization.

Mots clés: Life Sciences

Kedar Deshpande, Tangir Ahamed, Joop H. ter Horst, Peter T. Jansens, Luuk A.M. van Der Wielen, et al.. The use of Self Interaction Chromatography in stable formulation and crystallization of proteins. Biotechnology Journal, 2009, 4 (9), pp.1266. ⟨10.1002/biot.200800226⟩. ⟨hal-00498911⟩