Searching for proteins of subspecies with diagnostic potential by comparative qualitative proteomic analysis of mycobacterial tuberculins

Wiebren Santema , Marije Overdijk , Judith Barends , Jeroen Krijgsveld , Victor Rutten , Ad Koets

Accurate immunodiagnosis of bovine paratuberculosis is among others hampered by the lack of specific antigens. One of the most frequently used antigen preparations is purified protein derivative (PPD), also known as tuberculin. This crude extract has limitations when used in diagnostic assays due to the presence of cross-reactive antigens. The aim of the current study was to systematically analyze the qualitative protein composition of PPD of the major mycobacterial pathogens.

Mots clés: Tuberculins; Proteomics; Paratuberculosis; Antigen

Wiebren Santema, Marije Overdijk, Judith Barends, Jeroen Krijgsveld, Victor Rutten, et al.. Searching for proteins of subspecies with diagnostic potential by comparative qualitative proteomic analysis of mycobacterial tuberculins. Veterinary Microbiology, 2009, 138 (1-2), pp.191. ⟨10.1016/j.vetmic.2009.03.021⟩. ⟨hal-00490555⟩