Iron speciation study in knockout mice tissues: Magnetic and ultrastructural characterisation

Lucía Gutiérrez , Carmen Quintana , Cristina Patiño , Javier Bueno , Hélène Coppin , Marie P. Roth , Francisco J. Lázaro

Liver, spleen and heart tissues of DBA/2 knockout mice have been characterised by low temperature AC magnetic susceptibility measurements together with Transmission Electron Microscopy (TEM) and Selected Area Electron Diffraction in order to investigate the chemical iron speciation in a murine model of iron overload diseases. With emphasis on ferritin-like species, the temperature dependent in-phase and out-of-phase susceptibility profiles agree with the elemental analysis in that, in this model, iron accumulation takes place in the hepatic tissue while in the spleen and heart tissues no differences have been observed between knockout and wild type animals. The comparison of the magnetic properties between perfused and non-perfused liver tissues has made it possible to estimate the magnetic contribution of usually present blood remains. The TEM observations reveal that, besides the isolated ferritins and ferritin-containing lysosomes-siderosomes present in the hepatocytes, other iron deposits, of heterogeneous size, morphology and crystalline structure (haematite and/or goethite), are present in the cytoplasm, near the membrane, and in extracellular spaces.

Mots clés: Ferritin; Iron overload diseases; Hfe knockout; Magnetic susceptibility; Biomineralisation

Lucía Gutiérrez, Carmen Quintana, Cristina Patiño, Javier Bueno, Hélène Coppin, et al.. Iron speciation study in knockout mice tissues: Magnetic and ultrastructural characterisation. Biochimica et Biophysica Acta - Molecular Basis of Disease, 2009, 1792 (6), pp.541. ⟨10.1016/j.bbadis.2009.03.007⟩. ⟨hal-00488809⟩