Characterization of isolates from poultry, pigs, emus, the poultry red mite and other animals
Résumé
is the causative agent of erysipelas in mammals and birds, especially pigs and poultry. In order to investigate the suitability of different subtyping methods for genetic and phenotypic similarities among Swedish isolates of the organism, 45 isolates from poultry (=23), pigs (=17), emus (=2) and the poultry red mite (=3) were investigated by serotyping, pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Sequence analysis of the 16S rRNA gene was performed on eleven isolates from nine animal species. The results indicated a random scattering of serotypes throughout the dendrogram based on PFGE banding patterns following I digestion. In three cases, isolates with an identical PFGE pattern were of differing serotypes. No differentiation into subgroups by antimicrobial susceptibility testing by broth microdilution was possible as results were similar for all isolates. The Minimum Inhibitory Concentrations for most antimicrobials, including penicillin and oxytetracycline, were low. The 16S rRNA gene sequences (1443nts) from eight of eleven selected isolates of spp. were identical to that of the type strain ATCC 19414. The other three isolates differed from the type strain by two or three nucleotides. While this method may be useful for identification of spp., it is unsuitable for epidemiological investigations. Similarities in PFGE banding patterns between isolates from chickens and mites supported the hypothesis that may act as a reservoir and vector for . Further PFGE studies on isolates are appropriate to investigate the epidemiology of poultry erysipelas.
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