Plasma proteome changes in type 2 diabetes mellitus subjects with low or high early insulin response

Tea Sundsten (1) , Björn Zethelius , Christian Berne , Peter Bergsten

1 Department of Medical Cell Biology

Circulating proteins are in various ways contributing to the pathogenesis of type 2 diabetes mellitus (T2DM). The aim of the study was to investigate variations in plasma protein levels in persons with T2DM and differences in beta cell function, characterized by the early insulin response (EIR), and compare these protein levels with those observed in individuals with normal glucose tolerance (NGT). Ten individuals with NGT and high EIR, 10 with T2DM and high EIR and 10 with T2DM and low EIR were selected from the community-based Uppsala longitudinal study of adult men (ULSAM) cohort. Plasma protein profiling was performed using surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) MS. In total, nine plasma proteins were differing between the three study groups (ANOVA, p<0.05). Two forms of transthyretin, haemoglobin alpha-chain and haemoglobin beta-chain levels were decreased in plasma from T2DM persons compared to NGT individuals, irrespectively of the persons' EIR. Apolipoprotein H was decreased in plasma from individuals with T2DM and high EIR compared to persons with NGT. Four additional un-identified plasma proteins were also varying in different ways between the experimental groups. The proteins found in this study, may be related to the development of beta cell dysfunction.

Mots clés: Medicine

Tea Sundsten, Björn Zethelius, Christian Berne, Peter Bergsten. Plasma proteome changes in type 2 diabetes mellitus subjects with low or high early insulin response. Clinical Science, 2007, 114 (7), pp.499-507. ⟨10.1042/CS20070323⟩. ⟨hal-00479403⟩