Association between serum values of C-reactive protein and cytokine production in whole blood of patients with Type 2 diabetes
Résumé
Diabetes mellitus accelerates the atherosclerotic processes. It is known that inflammation plays a key role in atherosclerosis. The aim of the study was to evaluate in type 2 diabetic patients whether serum levels of C-reactive protein (CRP) is associated to cytokine production in whole blood. Eighty-nine type 2 diabetic outpatients were enrolled. Blood pressure, body mass index, fasting blood glucose, HbA1c, cholesterol, triglycerides and high sensitivity (hs)-CRP were measured. Interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured before and after 24 hours of incubation of whole blood with lipopolysaccharide (LPS) or saline solution. The basal values of IL-1β, IL-6 and TNF-α were low and not significantly related to hs-CRP levels. A univariate analysis showed that the level of IL-1β and IL-6, obtained after 24 hours of incubation of whole blood with LPS, significantly increased with increasing levels of hs-CRP and, after adjusting for potential confounders, IL-1β still remained statistically significant. In our population of type 2 diabetic patients there is no association between serum hs-CRP levels and the basal levels of IL-6, IL-1β and TNF-α. Conversely, a significant association was observed between serum hs-CRP levels and IL-1β and IL-6 production after 24 hours of incubation of whole blood with LPS. Our data suggest that type 2 diabetic patients with high hs-CRP values might have a major reactivity in response to specific stimuli to produce different interleukins, with possible implications in inflammatory- atherosclerotic processes.
Origine : Fichiers produits par l'(les) auteur(s)
Loading...