Target-assembled exciplexes based on Scorpion oligonucleotides

Abdul Gbaj , Lindsey Walsh , Maria C Rogert , Alireza Sardarian , Elena Vladimirova Bichenkova , Laura L Etchells , David Whitcombe , Kenneth T Douglas (1)

1 School of Pharmacy and Pharmaceutical Sciences

Scorpion probes, specific DNA probe sequences held in a hairpin loop can be modified to carry the components of an exciplex, for use as a novel fluorescence-based method for specific detection of DNA. The exci-partners (5'-pyrenyl and 3'-naphthalenyl) were attached to oligonucleotides via phosphoramidate links to terminal phosphate groups. Hybridisation of the probe to a complementary target in a buffer containing trifluoroethanol led to an obvious fluorescence change from blue (pyrene locally excited state emission) to green (exciplex emission).

Mots clés: Life Sciences

Abdul Gbaj, Lindsey Walsh, Maria C Rogert, Alireza Sardarian, Elena Vladimirova Bichenkova, et al.. Target-assembled exciplexes based on Scorpion oligonucleotides. Bioscience Reports, 2008, 28 (1), pp.1-5. ⟨10.1042/BSR20070006⟩. ⟨hal-00479291⟩