Hydrogen sulfide destroys lipid hydroperoxides in oxidized LDL

Markus K. Muellner , Sabine M. Schreier , Hilde Laggner (1) , Marcela Hermann , Harald Esterbauer , Markus Exner , Bernhard M.K. Gmeiner , Stylianos Kapiotis

1 IMC

Lipid hydroperoxides (LOOHs) in oxidized LDL (oxLDL) are potentially atherogenic compounds. Recently, H2S was identified as the third endogenous gasotransmitter in the vasculature. Hydrogen peroxide is known to be destroyed by H2S. Assuming that H2S may also react with LOOHs, the results show that H2S can destroy LOOHs in oxLDL. LOOH-enriched LDL after H2S-pretreatment was not further oxidized by endothelial cells and macrophages and the ability of oxLDL to induce HO-1 in endothelial cells was abolished by H2S pretreatment. HPLC analysis showed that 9-HPODE (9-hydroperoxy-octadecadienoic acid), a compound found in oxLDL, was reduced to 9-HODE (9-hydroxy-octadecadienoic acid) in presence of H2S. Thus, H2S may act as an antiatherogenic agent by reducing LOOHs to the less reactive LOHs and could abrogate the pathobiological activity of oxLDL.

Mots clés: Life Sciences

Markus K. Muellner, Sabine M. Schreier, Hilde Laggner, Marcela Hermann, Harald Esterbauer, et al.. Hydrogen sulfide destroys lipid hydroperoxides in oxidized LDL. Biochemical Journal, 2009, 420 (2), pp.277-281. ⟨10.1042/BJ20082421⟩. ⟨hal-00479143⟩