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| HAL : hal-00433273, version 1 |
| PubMed : 2658794 |
| PubMed Central : 172475 |
| Fiche détaillée |  Récupérer au format |
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| Antimicrobial Agents and Chemotherapy 33, 4 (1989) 535-40 |
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| Minimum antibiotic levels for selecting a resistance plasmid in a gnotobiotic animal model. |
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D. E. Corpet 1S. Lumeau 1 |
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| (04/1989) |
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| The minimum antibiotic concentrations for selecting an R plasmid in vivo were determined in germfree mice colonized by two isogenic strains of Escherichia coli, one of which carried an R plasmid. Seventy groups of three gnotobiotic mice were given low doses of ampicillin, colistin, flumequin, gentamicin, tetracycline, or streptomycin via drinking water for 2 weeks. The equilibrium between susceptible and resistant populations of bacteria was monitored daily in feces and compared with that of control mice given pure water. This model yielded reproducible data, and dose and response were strongly correlated. The minimum selecting doses ranged from 0.9 to 12.8 micrograms/ml of water, depending on the antibiotic and the R plasmid. The use of mathematical models and complementary in vitro experiments accounted for the effect of the low antibiotic levels. |
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| 1 : | Xénobiotiques |
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Institut national de la recherche agronomique (INRA) : UR1089 – Ecole Nationale Vétérinaire de Toulouse |
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| 2 : | Laboratoire de Génétique Cellulaire (LGC) |
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Institut national de la recherche agronomique (INRA) : UR0444 – Ecole Nationale Vétérinaire de Toulouse |
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| Domaine | : | Sciences du Vivant/Cancer |
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| Antimicrobial Agents – minimum antibiotic concentration – selection – R plasmid – in vivo – germfree – mouse – mice – Escherichia coli – gnotobiotic mice – ampicillin – colistin – flumequin – gentamicin – tetracycline – streptomycin – resistant bacteria – animal model – mathematical model – minimum selecting concentration – residues – food – risk assessment – veterinary treatment |
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| Liste des fichiers attachés à ce document : | |||||
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| hal-00433273, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00433273 | |
| oai:hal.archives-ouvertes.fr:hal-00433273 | |
| Contributeur : Denis Corpet | |
| Soumis le : Mercredi 18 Novembre 2009, 16:11:29 | |
| Dernière modification le : Mercredi 18 Novembre 2009, 17:11:09 | |
