Preparative scale enzymatic synthesis of D-sedoheptulose-7-phosphate from β-hydroxypyruvate and D-ribose-5-phosphate

Franck Charmantray (1) , Virgil Hélaine (1) , Bertrand Legeret (1) , Laurence Hecquet (1)

1 SEESIB - Synthèse et étude de systèmes à intêret biologique

An enzymatic method for ready access to D-sedoheptulose-7-phosphate on a preparative scale was developed, based on the irreversible transketolase-catalyzed reaction: β-hydroxypyruvate + D-ribose-5- phosphate → D-sedoheptulose-7-phosphate. D-Sedoheptulose-7-phosphate disodium salt was obtained in 81% overall yield determined using a standard curve obtained by LC/MS/MS.

Mots clés: D-Sedoheptulose-7-phosphate; Biocatalysis; Transketolase

Domaines: Catalyse

Franck Charmantray, Virgil Hélaine, Bertrand Legeret, Laurence Hecquet. Preparative scale enzymatic synthesis of D-sedoheptulose-7-phosphate from β-hydroxypyruvate and D-ribose-5-phosphate. Journal of Molecular Catalysis B: Enzymatic, 2009, 57, pp.6-9. ⟨hal-00366191⟩