Comparison of several commercial serologic kits and Em18 serology for detection of human alveolar echinococcosis.

Jean-Mathieu Bart , Martine Piarroux (1) , Yasuhito Sako , Fréderic Grenouillet (2) , Solange Bresson-Hadni (1, 2) , Renaud Piarroux (2) , Akira Ito (3, 4)

1 WHO Collaborating Center on Prevention and Treatment of Human Echinococcosis, SERF Unit
2 LCE - Laboratoire Chrono-environnement (UMR 6249)
3 FRCGC - Frontier Research Center for Global Change
4 AMC - Asahikawa Medical College, Dept of Parasitology

The aim of this study was to design the best serologic strategy for diagnosing human alveolar echinococcosis (AE) cases in medical laboratory routine procedures. By combining 2 screening techniques, indirect hemagglutination with Em(2plus)-ELISA and/or recEm18-ELISA, 46 of 47 AE cases were detected. The necessary confirmation of results is then obtained by using immunoblot (LDBIO-IB and/or recEm18-IB).

Domaines: Mycologie

Jean-Mathieu Bart, Martine Piarroux, Yasuhito Sako, Fréderic Grenouillet, Solange Bresson-Hadni, et al.. Comparison of several commercial serologic kits and Em18 serology for detection of human alveolar echinococcosis.. Diagnostic Microbiology and Infectious Disease, 2007, 59 (1), pp.93-5. ⟨10.1016/j.diagmicrobio.2007.03.018⟩. ⟨hal-00341437⟩