Monitoring the non-specific interactions of catechin through diffusion measurements based on pulsed-field gradients

C. Monteiro (1) , C. Maechling (2) , C. Herve Du Penhoat (1)

1 CERMAV - Centre de Recherches sur les Macromolécules Végétales
2 Laboratoire de Pharmacochimie de la Communication Cellulaire

The self-association of aqueous catechin as a function of concentration was monitored through variationsin 1H chemical shifts, proton T1 and T2 data and translational diffusion coefficients obtained with the pulsed-field gradient spin-echo method. The latter approach is very efficient and it is not restricted to aromatic compounds. Equilibrium constants were estimated for various models of self-association and the apparent enthalpy of dissociation was measured with isothermal titration calorimetry. Comparison of the latter parameter with thermodynamic data reported for various types of non-specific interactions suggests that such phenomena could be studied using this approach.

Mots clés: NMR; diffusion measurements; tannin; catechin; self-association; non-specific interactions; isothermal titration calorimetry; pulsed-field gradient spin-echo experiment

Domaines: Polymères

C. Monteiro, C. Maechling, C. Herve Du Penhoat. Monitoring the non-specific interactions of catechin through diffusion measurements based on pulsed-field gradients. Magnetic Resonance in Chemistry, 2002, pp.S110-S114. ⟨hal-00307293⟩