Quantification of the CBD-FITC conjugates surface coating on cellulose fibres

R. Pinto (1) , A.L. Amaral (1) , E.C. Ferreira (1) , M. Mota (1) , M. Vilanova (2) , K. Ruel (3) , M. Gama (2)

1 IBB - Institute for Biotechnology and Bioengineering
2 3ICBAS - Instituto de Ciências Biomédicas de Abel Salazar
3 CERMAV - Centre de Recherches sur les Macromolécules Végétales

Background: Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis. Results: For a CBD-FITC concentration of 60 mg/L, a coating fraction of 78% and 110% was estimated for Portucel and Whatman fibres, respectively. For a saturating CBD concentration, using Whatman CF11 fibres, a surface concentration of 25.2 × 10-13 mol/mm2 was estimated, the equivalent to 4 protein monolayers. This result does not imply the existence of several adsorbed protein layers. Conclusion: It was verified that CBDs were able to penetrate the fibres, according to confocal microscopy and TEM-immunolabelling analysis. The surface concentration of adsorbed CBDs was greater on amorphous fibres (phosphoric acid swollen) than on more crystalline ones (Whatman CF11 and Sigmacell 20).

Domains: Polymers

R. Pinto, A.L. Amaral, E.C. Ferreira, M. Mota, M. Vilanova, et al.. Quantification of the CBD-FITC conjugates surface coating on cellulose fibres. BMC Biotechnology, 2008, pp.1-7. ⟨hal-00303871⟩