The use of high halide-ion concentrations and automated phasing procedures for the structural analysis of BclA, the major component of the exosporium of Bacillus anthracis spores.

Sylvie Salamitou (1) , Stéphane Réty (2, 3) , Françoise Le Hégarat (1) , Gérard Leblon (1) , Anita Lewit-Bentley (2, 3)

1 IGM - Institut de génétique et microbiologie [Orsay]
2 LBPA - Laboratoire de Biologie et de Pharmacologie Appliquée
3 LURE - Laboratoire pour l'utilisation du rayonnement électromagnétique

The structure determination of the recombinant form of BclA, the major protein component of Bacillus anthracis exosporium, involved soaking in a high concentration of potassium iodide as the means of obtaining a good-quality heavy-atom derivative. The data to 2 angstroms resolution collected on a laboratory source were of sufficient quality to allow successful phasing and chain tracing by automated methods.

Mots clés: Crystal structure; Automated phasing; Halide ions

Domaines: Biologie structurale [q-bio.BM]

Sylvie Salamitou, Stéphane Réty, Françoise Le Hégarat, Gérard Leblon, Anita Lewit-Bentley. The use of high halide-ion concentrations and automated phasing procedures for the structural analysis of BclA, the major component of the exosporium of Bacillus anthracis spores.. Acta crystallographica Section D : Structural biology [1993-..], 2005, 61 (Pt 3), pp.344-9. ⟨10.1107/S090744490403416X⟩. ⟨hal-00287092⟩