Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy

Ching-Ting Lin (1) , En-Kuang Tien (1) , Szu-Yuan Lee (1) , Long-Sheng Lu (2) , Chau-Chung Wu (3) , Chen-Yuan Dong (3) , Chii-Wann Lin (1)

1 Institute of Biomedical Engineering
2 College of medicine
3 Dep. of Physics

Ox-LDL uptakes by macrophage play a critical role in the happening of atherosclerosis. Because of its low damage on observed cells and better signal-to- background ratio, two-photon excitation fluorescence microscopy is used to observe NAD(P)H autofluorescence of macrophage under difference cultured conditions- bare cover glass, coated with fibronectin or poly-D-lysine. The results show that the optimal condition is fibronectin coated surface, on which, macrophages profile can be clearly identified on NAD(P)H autofluorescence images collected by two-photon microscopy. Moreover, different morphology and intensities of autofluorescence under different conditions were observed as well. In the future, effects of ox-LDL on macrophages will be investigated by purposed system to research etiology of atherosclerosis.

Mots clés: Two-photon microscopy; atherosclerosis; ox-LDL; autofluorescence

Domaines: Biophysique [physics.bio-ph]

Ching-Ting Lin, En-Kuang Tien, Szu-Yuan Lee, Long-Sheng Lu, Chau-Chung Wu, et al.. Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy. ENS 2005, Dec 2005, Paris, France. pp.30-36. ⟨hal-00166980⟩