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Table 2.

mass spectrometry analysis of various protein spots

Spot Number identification Accession number Detection method Sequence coverage Number of matched peptides
1 Band 3 Anion Transport Protein P02730 RuBPS 32% 26
Theoret Mw/pI: 101727/5,08 Ag 17% 16
Ag-NH3 30% 30
2 Protein 4.1 P11171 RuBPS 34% 33
Theoret Mw/pI: 96957/5,45 Ag 38% 32
Ag-NH3 39% 38
3 Protein 4.2 P16542 RuBPS 21% 13
Theoret Mw/pI: 76794/8.27 Ag NA 0
Ag-NH3 NA 0
4 GAPDH P04406 RuBPS 57% 24
Theoret Mw/pI: 35899/8,58 Ag 69% 26
Ag-NH3 68% 27
5 dematin Q08495 RuBPS 49% 21
Theoret Mw/pI: 45486/8,94 Ag 33% 31
Ag-NH3 46% 21
6 Spectrin alpha chain P02549 RuBPS 34% 89
Theoret Mw/pI: 279744/4,96 Ag 30% 72
Ag-NH3 25% 65
7 Spectrin beta chain P11277 RuBPS 12% 26
Theoret Mw/pI: 246170/5,13 Ag NA 0
Ag-NH3 NA 0
8 Rho GDI 2 Q61599 Ru-BPS 24% 5
Theoret Mw/pI: 22836/4,97 Ag NA 0
Ag-NH3 21% 4
Shev 36% 7
9 PGM P18669 Ru-BPS 64% 14
Theoret Mw/pI: 28655/6,75 Ag 31% 7
Ag-NH3 43% 10
Shev 37% 9
10 Ran P17080 Ru-BPS 30% 7
Theoret Mw/pI: 24408/7,01 Ag NA 0
Ag-NH3 24% 6
Shev ND ND
11 Alpha enolase P17182 Ru-BPS 58% 23
Theoret Mw/pI: 46980/6,36 Ag 22% 9
Ag-NH3 38% 15
Shev 36% 13
12 PDI P17182 Ru-BPS 49% 29
Theoret Mw/pI: 56586/5,98 Ag 19% 10
Ag-NH3 19% 10
Shev 33% 13
13 endoplasmin P08113 Ru-BPS 32% 29
Theoret Mw/pI: 92418/4,74 Ag NA 0
Ag-NH3 23% 24
Shev 19% 18
14 Hsp 90 beta P08238 Ru-BPS 35% 27
Theoret Mw/pI: 83081/4,97 Ag 10% 8
Ag-NH3 21% 15
Shev 33% 25

Ru-BPS: ruthenium complex fluorescent staining; Ag: silver nitrate staining [10]. Ag-NH3: silverammonia staining (table 1); Shev: silver nitrate staining according to Shevchenko et al. [12].

NA: not available (no identification). ND: not detected by the method selected

Proteomics. 2006 April; 6(8): 2350–4.
doi: 10.1002/pmic.200500567.