mass spectrometry analysis of various protein spots
mass spectrometry analysis of various protein spots
Spot Number | identification | Accession number | Detection method | Sequence coverage | Number of matched peptides |
---|---|---|---|---|---|
1 | Band 3 Anion Transport Protein | P02730 | RuBPS | 32% | 26 |
Theoret Mw/pI: 101727/5,08 | Ag | 17% | 16 | ||
Ag-NH3 | 30% | 30 | |||
2 | Protein 4.1 | P11171 | RuBPS | 34% | 33 |
Theoret Mw/pI: 96957/5,45 | Ag | 38% | 32 | ||
Ag-NH3 | 39% | 38 | |||
3 | Protein 4.2 | P16542 | RuBPS | 21% | 13 |
Theoret Mw/pI: 76794/8.27 | Ag | NA | 0 | ||
Ag-NH3 | NA | 0 | |||
4 | GAPDH | P04406 | RuBPS | 57% | 24 |
Theoret Mw/pI: 35899/8,58 | Ag | 69% | 26 | ||
Ag-NH3 | 68% | 27 | |||
5 | dematin | Q08495 | RuBPS | 49% | 21 |
Theoret Mw/pI: 45486/8,94 | Ag | 33% | 31 | ||
Ag-NH3 | 46% | 21 | |||
6 | Spectrin alpha chain | P02549 | RuBPS | 34% | 89 |
Theoret Mw/pI: 279744/4,96 | Ag | 30% | 72 | ||
Ag-NH3 | 25% | 65 | |||
7 | Spectrin beta chain | P11277 | RuBPS | 12% | 26 |
Theoret Mw/pI: 246170/5,13 | Ag | NA | 0 | ||
Ag-NH3 | NA | 0 | |||
8 | Rho GDI 2 | Q61599 | Ru-BPS | 24% | 5 |
Theoret Mw/pI: 22836/4,97 | Ag | NA | 0 | ||
Ag-NH3 | 21% | 4 | |||
Shev | 36% | 7 | |||
9 | PGM | P18669 | Ru-BPS | 64% | 14 |
Theoret Mw/pI: 28655/6,75 | Ag | 31% | 7 | ||
Ag-NH3 | 43% | 10 | |||
Shev | 37% | 9 | |||
10 | Ran | P17080 | Ru-BPS | 30% | 7 |
Theoret Mw/pI: 24408/7,01 | Ag | NA | 0 | ||
Ag-NH3 | 24% | 6 | |||
Shev | ND | ND | |||
11 | Alpha enolase | P17182 | Ru-BPS | 58% | 23 |
Theoret Mw/pI: 46980/6,36 | Ag | 22% | 9 | ||
Ag-NH3 | 38% | 15 | |||
Shev | 36% | 13 | |||
12 | PDI | P17182 | Ru-BPS | 49% | 29 |
Theoret Mw/pI: 56586/5,98 | Ag | 19% | 10 | ||
Ag-NH3 | 19% | 10 | |||
Shev | 33% | 13 | |||
13 | endoplasmin | P08113 | Ru-BPS | 32% | 29 |
Theoret Mw/pI: 92418/4,74 | Ag | NA | 0 | ||
Ag-NH3 | 23% | 24 | |||
Shev | 19% | 18 | |||
14 | Hsp 90 beta | P08238 | Ru-BPS | 35% | 27 |
Theoret Mw/pI: 83081/4,97 | Ag | 10% | 8 | ||
Ag-NH3 | 21% | 15 | |||
Shev | 33% | 25 |