PubMed Central, Table 1: Proteomics. 2006 April; 6(8): 2350

flowchart for silver ammonia staining

Step	solution	Time
Fixation	Ethanol 30% (v/v) Acetic acid 10% (v/v) 2,7 Naphthalene disulfonic acid 0.05%	overnight
Rinse	water	6× 10 minutes
Silvering	Silver nitrate 24 mM, NaOH 15mM, ammonium hydroxide 75mM	20–30 minutes
Rinse	water	3 × 5 minutes
development	37% formaldehyde: 1 ml/l, citric acid 80 mg/1	5–10 minutes
Stop	Ethanolamine 5 ml/l, acetic acid 20 ml/l	30 minutes

Polymerization initiator for inclusion of thiosulfate in gels: use 0.7μl TEMED, 6μl of 10% sodium thiosulfate solution and 7μl of 10% ammonium persulfate solution (to be added last) per ml of gel solution