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Isolation and characterization of Phanerochaete chrysosporium mutants resistant to antifungal compounds

Abstract : Ligninolytic fungi are wood-degrading organisms that use cellulose, hemicelluloses, and lignin as sources of carbon and energy. However, the oxidative processes used by these fungi to decompose wood release a myriad of potentially toxic extractable molecules. These extractives are non-structural components of wood. They are involved in wood properties such as color, odor, and natural durability as they have antifungal properties. In order to adapt to this toxic environment, the fungi which are responsible for decomposing wood have developed various strategies to detoxify the antifungal molecules. In order to study this detoxification, we have developed a direct genetic strategy in Phanerochaete chrysosporium known as the wood-decomposing fungus model. After performing UV mutagenesis, screens allowing the identification of fungal strains resistant to different antifungal agents were carried out. Two selected molecules, including itraconazole and rapamycin with known mechanisms of antifungal action, made it possible to obtain two first mutant collections that were characterized. Next, extractives from two wood species (Bagassa guianensis and Prunus avium) with antifungal activities were used to produce two collections of mutants resistant to these extractives. The characterization of these mutants should allow us to understand why these extractives are toxic and how the fungus is able to detoxify them. The possibility of occurring studies on ligninolytic fungi opens new perspectives. Indeed, these fungi have been the subject of growing interest in recent years, mainly because of their potential use in the valorization of biomass to produce biofuels, their important function in the global carbon cycle, and also because of their wood material damage.
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Submitted on : Wednesday, September 16, 2020 - 9:54:43 AM
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Duy Vuong Nguyen. Isolation and characterization of Phanerochaete chrysosporium mutants resistant to antifungal compounds. Mycology. Université de Lorraine, 2020. English. ⟨NNT : 2020LORR0045⟩. ⟨tel-02940144⟩

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