, Et pour conclure, mes frères, je citerai Saint Jean, dans son Epître aux Chercheurs, Livre LXXIII, vol.8, pp.2-3

, Et le 15 éme jour, épuisé par une dure semaine

, Se demandant quelle utilité cette protéine pourrait bien avoir dans son

. Oeuvre, . Dans-une-solution-saline, M. Effroi-qu-;-»-references-budayova-spano, F. Bonneté, J. P. Astier et al., elle s'auto-associait. Stupéfait d'horreur et devinant un méfait du Malin, il décida d'enfouir la protéine diabolique au plus profond du plus épais des animaux : le pancréas du bovin, là où les Hommes ne pourraient jamais l'atteindre. Rasséréné, il décida alors de s'accorder un jour de congé supplémentaire, J. Cryst. Growth, vol.235, pp.547-554, 2002.

C. Boulin, R. Kempf, M. H. Koch, and S. M. Mclaughlin, Nucl. Instrum. Methods A, vol.249, pp.399-407, 1986.

C. Depautex, C. Desvignes, P. Feder, M. Lemonnier, R. Bosshard et al., LURE: rapport d'activité pour la période Août, 1985.

J. M. Dubuisson, T. Decamps, and P. Vachette, J. Appl. Cryst, vol.30, pp.49-54, 1997.

O. Galkin and P. G. Vekilov, Proc. Natl Acad. Sci USA, vol.97, pp.6277-6281, 2000.

C. Haas and J. Drenth, J. Cryst. Growth, vol.196, pp.388-394, 1999.

C. Hamiaux, T. Prangé, M. Riès-kautt, A. Ducruix, S. Lafont et al., Acta Cryst, vol.55, pp.103-113, 1999.

C. Hamiaux, , 2000.

C. Hamiaux, J. Perez, T. Prangé, S. Veesler, M. Riès-kautt et al., J. Mol. Biol, vol.297, pp.697-712, 2000.

S. Lafont, S. Veesler, J. P. Astier, and R. Boistelle, J. Cryst. Growth, vol.173, pp.132-140, 1997.

V. Luzzati and A. Tardieu, Ann. Rev. Biophys. Bioeng, vol.9, pp.1-29, 1980.

M. Muschol and F. Rosenberger, J. Chem. Phys, vol.107, pp.1953-1961, 1997.

D. Svergun, C. Barberato, and M. H. Koch, J. Appl. Cryst, vol.28, pp.768-773, 1995.

A. Tardieu, Neutron and synchrotron radiation for condensed matter studies, vol.III, pp.145-160, 1994.

P. Vachette, F. Strasbourg, S. Veesler, S. Marcq, S. Lafont et al., Acta Cryst, vol.50, pp.355-360, 1979.

P. R. Wolde and D. Frenkel, Science, vol.277, pp.1975-1978, 1997.

F. A. Bibliographie-anderer and . Hornle-s, Moleculargewicht, endgruppenanalyse, und aminosaure-zusammensetzung, Strukturuntersuchungen am kallikrein-inaktivator aus rinderlunge I, pp.457-462, 1965.

. A. Anderer-f and . Hornle-s, The disulfide linkages in kallikrein inactivator of bovine lung, The J. of Biological Chemistry, vol.241, issue.7, pp.1568-1572, 1965.

T. Arakawa and . N. Timasheff-s, theory of protein solubility, Methods in enzymology, vol.114, pp.49-77, 1985.

. Asherie-n, J. Pande, . Lomakin-a, O. Ogun, . R. Hanson-s et al., Oligomerization and phase separation in globular protein solutions, Biophysical Chemistry, vol.75, pp.213-227, 1998.

J. P. Astier, D. Bokern, L. Lapena, and . Veesler-s, a-amylase crystal growth investigated by in situ atomic force microscopy, J. Cryst. Growth, vol.226, pp.294-302, 2001.

M. Ataka and A. , Systematic studies on the crystallization of lysozyme, J. Cryst. Growth, vol.90, pp.86-93, 1988.

. Belloni-l, Electrostatic interactions in colloidal solutions: comparison between primitive and one-component models, J. Chem. Phys, vol.85, issue.1, pp.519-526, 1986.

. Belloni-l, Self-consistent integral equation applied to the highly charged primitive model, J. Chem. Phys, vol.88, issue.8, pp.5143-5148, 1988.

. Belloni-l, Interacting monodisperse and polydisperse spheres. Neutron, X-Ray and light scattering, D. Series. Amsterdam, pp.135-155, 1991.

. Belloni-l, Diffusion de la lumière, 1994.

P. Benas, L. Legrand, and R. , Strong and specific effects of cations on lysozyme chloride solubility, Acta Cryst. D, vol.58, pp.1582-1587, 2002.

. J. Berne-b and . Pecora-r, Dynamic light scattering with applications to chemistry, biology, and physics, 1976.

. Boistelle-r, Concepts de la cristallisation en solution. Actualités néphrologiques, Flamarion medecine sciences, pp.159-202, 1985.

R. Boistelle and A. P. , Crystallization mechanisms in solution, J. Cryst. Growth, vol.90, pp.14-30, 1988.

. Boistelle-r, J. P. Astier, . Marchis-mouren-g, V. Desseaux, and . Haser-r, , 1992.

. Solubility, phase transition, kinetic ripening and growth rates of porcine pancreatic a-amylase isoenzymes, J. Crystal Growth, vol.123, pp.109-120

. Bonnete-f, N. Ferte, J. P. Astier, and . Veesler-s, Protein crystallization:contribution of small angle X ray scattering, Journal de Physique IV, vol.118, pp.3-13, 2004.

. Bonnete-f, S. Finet, and . Tardieu-a, Second virial coefficient: variations with lysozyme crystallization conditions, Journal of Crystal Growth, vol.196, issue.2-4, pp.403-414, 1999.

M. Bostrom, . R. Williams-d, and . W. Ninham-b, Specific ion effects: Why DLVO theory fails for biology and colloid systems, Phys. Rev. Lett, vol.87, issue.16, pp.168103-168104, 2001.

. L. Broide-m, C. R. Berland, J. Pande, . O. Ogun-o, and . B. Benedek-g, Binaryliquid phase separation of lens protein solutions, Proc. Natl. Acad. Sci. USA, vol.88, pp.5660-5664, 1991.

. L. Broide-m, . M. Tominc-t, and . D. Saxowsky-m, Using phase transitions to investigate the effect of salts on protein interactions, Physical Review. E. Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics, vol.53, issue.6, pp.6325-6335, 1996.

. Budayova-m, Cristallisation et caractérisation de protéines en solution : diagrammes de phases, cinétiques de croissance et associations moléculaires, 1998.

. G. Cacace-m, . M. Landau-e, and . J. Ramsden-j, The Hoffmeister series: salt and solvent effects on interfacial phenomena, Q. Rev. Biophys, vol.30, pp.241-277, 1997.

J. W. Cahn and H. J. , Free energy of a nonuniform system. III. Nucleation in a two-component incompressible fluid, The Journal of chemical physics, vol.31, issue.3, pp.688-699, 1959.

. D. Collins-k and . W. Washabaugh-m, The Hoffmeister effect and the behaviour of water at interfaces, Q. Rev. Biophys, vol.18, issue.4, pp.323-422, 1985.

. H. Crick-f, The unit cells of four proteins, Acta Crystallogr, vol.6, pp.221-222, 1953.

U. A. Curtis-r, . Montaser-a, J. M. Prausnitz, and B. H. , , 2002.

, Protein-protein interactions in concentrated electrolyte solutions, Biotechnol. Bioeng, vol.79, issue.4, pp.367-380

M. Delaye, . E. Danford-kaplan-m, J. I. Clark, B. Krop, T. Gulik-krzywicki et al., Effect of calcium on the calf lens cytoplasm, Exp Eye Res, vol.44, issue.5, pp.601-617, 1987.

M. Delaye and . Tardieu-a, Short-range order of crystallin proteins accounts for eye lens transparency, Nature, vol.302, issue.31, pp.415-417, 1983.

C. Depautex, C. Desvignes, . Feder-p, M. Lemonnier, . Bosshart-r et al.,

P. Dagneaux, D. Benoit, and J. P. Vachette-p, LURE: rapport d'activité pour la période Aout, 1985.

J. Diesenhofer and . Steigemann-w, Crystallographc refinement of the the structure of bovine pancreatic trypsin inhibitor at 1.5 A resolution, Acta. Cryst. B, vol.31, pp.238-250, 1975.

J. M. Dubuisson, T. Decamps, and . Vachette-p, Improved signal-to-background ratio in small angle X-ray scattering experiments with synchrotron radiation using an evacuated cell for solutions, J. Appl. Cryst, vol.30, pp.49-54, 1997.

A. Ducruix and . Giege-r, Crystallization of nucleic acidsand protéins -a practical approach, 1992.

A. Ducruix and R. , Solubilisation, solubilité, diagrammes de phase: application à la cristallisation des proteines, Les cahiers Imabio, vol.15, 1995.

C. Ebel, P. Faou, and . Zaccai-g, Protein-solvent and weak protein-protein interactions in halophilic malate dehydrogenase, J. Cryst. Growth, vol.196, issue.2-4, pp.395-402, 1999.

C. Eigenbrot and R. M. Kossiakoff-a, Protein Data Bank entry 9PTI, Brookhaven National Laboratory, 1991.

M. Farnum and . Zukoski-c, Effect of glycerol on the interactions and solubility of BPTI, Biophysical Journal, vol.76, pp.2716-2726, 1999.

. Finet-s, F. Bonnete, J. Frouin, K. Provost, and . Tardieu-a, Lysozyme crystal growth, as observed by small angle X-ray scattering, proceeds without crystallization intermediates, EUR. BIOPHYS. J, vol.27, pp.263-271, 1998.

. Galkin-o, . Chen-k, . E. Nagel-r, and . G. Vekilov-p, Liquid-Liquid separation in solutions of normal and sickle cell hemoglobin, PNAS, vol.99, issue.13, pp.8479-8483, 2002.

O. Galkin and . G. Vekilov-p, Direct determination of the nucleation rates of protein crystals, J. Phys. Chem. B, vol.103, pp.10965-10971, 1999.

O. Galkin and . G. Vekilov-p, Control of protein crystal nucleation around the metastable liquid-liquid phase boundary, PNAS, vol.97, issue.12, pp.6277-6281, 2000.

O. Galkin and . G. Vekilov-p, Are nucleation kinetics of protein crystals similar to those of liquid droplets?, J. Am. Chem. Soc, vol.122, pp.156-163, 2000.

O. Galkin and . G. Vekilov-p, Nucleation of protein crystals: critical nuclei, phase behavior, and control pathways, J. Cryst. Growth, vol.232, pp.63-76, 2001.

. H. Gallagher-w and . K. Woodward-c, The concentration dependence of the diffusion coefficient for BPTI: a dynamic light scattering study of a small protein, Biopolymers, vol.28, pp.2001-2024, 1989.

. M. Garcia-ruiz-j, . L. Novella-m, R. Moreno, and . A. Gavira-j, Agarose as crystallization media for proteins I: Transport processes, J. Cryst. Growth, vol.232, pp.165-172, 2001.

Y. Georgalis, P. Umbach, . M. Soumpasis-d, and . Saenger-w, Dynamics and microstructure formation during nucleation of lysozyme solutions, J. Am. Chem. Soc, vol.120, pp.5539-5548, 1998.

Y. Georgalis, . Zouni-a, W. Eberstein, and . Saenger-w, Formation dynamics of protein precrystallization fractal clusters, J. Cryst. Growth, vol.126, pp.245-260, 1993.

G. A. Wilson-w, Predicting protein crystallisation from a dilute solution property, Acta Cryst. D, vol.50, pp.361-365, 1994.

. Gottschalk-m, K. Venu, and . Halle-b, Protein self association in solution: The BPTI decamer, Biophysical Journal, vol.84, pp.3941-3958, 2003.

. A. Green-a, VIII. The solubility of hemoglobin in concentraed salt solutions. A study of the salting out of proteins, Studies in the physical chemistry of the proteins, pp.495-516, 1931.

. A. Green-a, X. The solubility of hemoglobin in solutions of chlorides and sulfates of varying concentration, Studies in the physical chemistry of the proteins, pp.47-66, 1932.
URL : https://hal.archives-ouvertes.fr/in2p3-00025132

. Grillo-i, Small-angle neutron scattering study of a world-wide known emilsion: le Pastis, Colloids and surfaces A: Physicochem. Eng. Aspects, vol.225, pp.153-160, 2003.

. Grouazel-s, J. Perez, J. P. Astier, F. Bonnete, and . Veesler-s, BPTI liquid-Liquid phase separation monitored by light and small angle X-ray scattering, Acta Cryst. D, vol.58, pp.1560-1563, 2002.

E. A. Guggenheim, Thermodynamics. An advanced treatment for chemists and physicists, 1959.

A. Guinier, F. Wiley, . Gulik-krzywicki-t, A. Tardieu, and . Delaye-m, Spatial reorganization of lowmolecular-weight proteins during cold cataract opacification, Biochim Biophys Acta, vol.800, issue.1, pp.28-32, 1955.

C. Haas and D. , Understanding protein crystallisation on the basis of the phase diagram, J. Cryst. Growth, vol.196, pp.388-394, 1999.

C. Hamiaux, Cristallogenèse du BPTI à pH acide: étude des relations entre l'état d'association des molécules en solution et à l'état cristallin par diffusion et diffraction des rayons X, 2000.

P. Orsay,

C. Hamiaux, J. Perez, T. Prange, S. Veesler, M. Ries-kautt et al., The BPTI decamer observed in acidic pH crystal forms pre-exists as a stable species in solution, J. Mol. Biol, vol.297, pp.697-712, 2000.
URL : https://hal.archives-ouvertes.fr/hal-01745681

C. Hamiaux, T. Prange, . Ries-knaut-m, . Ducruix-a, S. Lafont et al., The decameric structure of BPTI crystallized from thiocyanate at 2.7 A resolution, Acta Cryst. D, vol.55, pp.103-113, 1999.
URL : https://hal.archives-ouvertes.fr/hal-01745648

. Hillert-m, Phase equilibria, phase diagrams and phase transfomations, 1998.

. Hoffmeister-f, Zur lehre von der wirkung der salze, Arch. Exp. Pathol. Pharmakol, vol.24, pp.247-260, 1888.

. B. Howard-s, . J. Twigg-p, J. K. Baird, and . J. Meehan-e, The solubility of hen eggwhite lysozyme, J. Cryst. Growth, vol.90, pp.94-104, 1988.

. Huber-r, . Kukla-a, . Ruhlmann-a, A. Epp, and . Formanek-h, The basic trypsin inhibitor of bovine pancreas, Naturwissenschaften, vol.57, pp.389-392, 1970.

. Ilyna-e, V. Roongta, . Pan, C. Woodward, and . H. Mayo-k, A pulsed field gradient NMR study of BPTI self association, Biochemistry, vol.36, pp.3383-3388, 1997.

C. Ishimoto and . Tanaka-t, Critical behavior of a binary mixture of protein and salt water, Phys. Rev. Lett, vol.39, issue.8, pp.474-477, 1977.

J. Israelachvili, Intermolecular and surface forces, 1994.

J. Israelachvili and . Wennerstrom-h, Role of hydratation and waterstructure in biological and colloidal interactions, Nature, vol.379, pp.219-225, 1996.

J. N. Israelachvili, Intermolecular and surface forces, with applications to colloidal and biological systems, 1985.

B. Kassell and . Laskowski-m, The basic trypsin inhibitor of bovine pancreas V. the disulfide linkages, Biochemical and biophysical reaserch communications, vol.20, issue.4, pp.463-468, 1965.

H. Kraut, E. Frey, and W. E. , Uber die inaktivierung des kallikreins, Hoppe Seylers Z. Physiol. Chem, vol.192, pp.1-21, 1930.

H. Kraut, W. Scholtan, and . Schultz-f, Versuche zur isolierung des kallikreininaktivators, III. Elektrophoretische reinung und molekulargewichts-bestimmung in der ultrazentrifuge, Hoppe Seylers Z. Physiol. Chem, vol.321, pp.90-96, 1960.

M. Kunitz and N. J. , Isolation from beef pancreas of crystalline trypsinogen, tripsin, a trypsin inhibitor and a inhibitor-trypsin compound, J. Gen. Physiol, vol.19, pp.991-1007, 1936.

Y. G. Kuznetsov, M. J. Malkin-a, and . Pherson-a, The liquid protein phase in crystallization : a case study-intact immunoglobulins, J. Crystal Growth, vol.232, pp.30-39, 2001.

. Lafferere-l, C. Hoff, and . Veesler-s, Study of liquid-liquid demixing from drug solution, J. Cryst. Growth, vol.269, pp.550-557, 2004.

. Lafont-s, Interactions des molécules de protéines en solution, application à la cristallogenèse, 1996.

. Lafont-s, S. Veesler, J. P. Astier, and . Boistelle-r, Solubility and prenucleation of aprotinin (BPTI) molecules in sodium chloride solutions, J. Crystal Growth, vol.14, pp.249-255, 1994.

. Lafont-s, S. Veesler, J. P. Astier, and . Boistelle-r, Prenucleation and crystallisation of proteins : Application to BPTI Solutions, 1996.

. Lafont-s, S. Veesler, J. P. Astier, and . Boistelle-r, Comparison of solubilities and molecular interactions of BPTI molecules giving different polymorphs, J. Crystal Growth, vol.173, pp.132-140, 1997.

H. N. Lekkerkerker, . Poon-w.c.-k, . N. Pusey-p, A. Stroobants, and . Warren,

P. B. , Phase behaviour of colloid+polymer mixtures, Europhysics letters, vol.20, issue.6, pp.559-564, 1992.

P. Linder and Z. Th, Neutron, X-ray and light scattering: introduction to an investigative tool for colloidal and polymeric systems, 1991.

. H. Lupis-c, Chemical thermodynamics of materials, 1983.

. Lutterbach-n, H. Versmold, V. Reus, L. Belloni, and . Zemb-t, Charge stabilized liquidlike ordered binary colloidal suspensions. 1. Ultra-Small-Angle X-Ray scattering characterization, Langmuir, vol.15, pp.337-344, 1999.

. Lutterbach-n, H. Versmold, L. Reus-v, Z. T. Belloni, and . Linder-p, , 1999.

, Charge stabilized liquidlike ordered binary colloidal suspensions. 2. Partial structure factors determined by small-angle neutron scattering, Langmuir, vol.15, pp.345-352

M. Malfois, F. Bonnete, L. Belloni, and . Tardieu-a, A model of attractive interactions to account for fluid-fluid phase separation of protein solutions, J. Chem. Phys, vol.105, issue.8, pp.3290-3300, 1996.

. J. Malkin-a, J. Cheung, and M. C. Pherson-a, Crystallisation of satellite tobacco mosaic virus I. Nucleation phenomena, J. Cryst. Growth, vol.126, pp.544-554, 1993.

M. J. Malkin-a and . Pherson-a, Light scattering investigation of protein and virus crystal growth: ferritin, apoferritin and satellite tobacco mosaic virus, J. Cryst. Growth, vol.128, pp.1232-1235, 1993.

M. J. Malkin-a and . Pherson-a, Crystallisation of satellite tobacco mosaic virus II. Postnucleation events, J. Cryst. Growth, vol.126, pp.555-564, 1993.

M. J. Malkin-a and . Pherson-a, Light scattering investigation of nucleation processes and kinetics of crystallization in macrmolecular systems, Acta Cryst. D, vol.50, pp.385-395, 1994.

. Marcelja-s, Hydratation in electrical double layers, Nature, vol.38, pp.689-690, 1997.

A. Mc-pherson, A. Malkin, and . G. Kutznetsov-y, Atomic force microscopy in the study of macromolecular crystal growth, Annu. Rev. Biophys. Biomol. Struct, vol.29, pp.361-410, 2000.

W. Melander and . Horvath-c, Salt effect on hydrophobic interactions in precipitation and chromatography of proteins: an interpretation of the lyotropic series, Arch. Biochem. Biophys, vol.183, issue.1, pp.200-215, 1977.

M. E. and H. , BPTI as unusual thermodynamic stability parameters, J. Mol. Biol, vol.170, pp.765-776, 1983.

M. Muschol and R. , L-L phase separation in supersaturated lysozyme solutions and associated precipitate formation/crystallisation, J. Chem. Phys, vol.107, issue.6, pp.1953-1961, 1997.

. E. Nielsen-a and . Sohnel-o, Interfacial tensions electrolyte crystal-aqueous solution from nucleation data, J. Cryst. Growth, vol.11, pp.233-242, 1971.

. Niimura-n, Y. Minezaki, M. Ataka, and . Katsura-t, Small angle neutron scattering from lysozyme in unsaturated solutions, to characterize the pre-crystallization process, J. Cryst. Growth, pp.671-675, 1994.

N. J. Kuntz and M. M. Herriot-r, Crystalline Enzymes, 1948.

. Ostwald-w, Studien uber die bildung und umwandlung fester korper, Z Physik Chem, vol.22, pp.289-330, 1897.

P. K. Pathria, Statistical Mecanics, pp.405-407, 1996.

. Pernodet-n, M. Maaloum, and . Tinland-b, Pore size of agarose gels by atomic force microscopy, Electrophoresis, vol.18, pp.55-58, 1997.

. Piazza-r, M. Pierno, and . Vignati-e, Liquid-liquid phase separation of a surfactantsolubilized membrane protein, PRL, vol.90, issue.20, pp.208101-208102, 2003.

W. H. Press, Numerical recipes -The art of scientific computing, 1986.

P. L. Privalov, Stability of proteins -smal globular proteins, Advances in protein chemistry, vol.33, pp.167-241, 1979.

. Pullara-f, . Emanuele-a, . B. Palma-vittorelli-m, and . U. Palma-m, , 2005.

, Lysozyme cristallization rates controlled by anomalous fluctuations, J. Cryst . Growth, vol.274, pp.536-544

. L. Pusey-m and . Gernet-k, A method for rapid liquid-solid phase solubility measurements using the protein lysozyme, J. Cryst. Growth, vol.88, pp.419-424, 1988.

. L. Pusey-m and . Gernet-k, Estimation of the initial equilibrium constants in the formation of tetragonal lysozyme nuclei, J. Cryst. Growth, vol.110, pp.60-65, 1991.

. Riess-kautt-mm and . F. Ducruix-a, Relative effectiveness of various ions on the solubility and crystal growth of lysozyme, J. Biol. Chem, vol.264, issue.2, pp.745-753, 1989.

. Riess-kautt-mm and . F. Ducruix-a, From solutions to crystals with a physicochemical aspect, 1999.

R. C. Neal-b and . M. Lenhoff-a, Van der Waals interactions betwenn proteins, Biophys. J, vol.70, pp.977-987, 1996.

W. Scholtan and . Y. Lie-s, Die makromolekulare chemie, vol.98, pp.204-234, 1966.

H. E. Stanley, Introduction to phase transitions and critical phenomena, p.42, 1971.

. Stradner-a, H. Sedwick, F. Cardinaux, . C. Poon-w, . U. Egelhaaf-s et al., Equilibrium cluster formation in concentrated protein solutions and colloids, Nature, vol.432, pp.492-495, 2004.

. Svergun-d, C. Barberato, and . H. Koch-m, CRYSOL -a program to evaluate X ray solution scattering of biological macromolecules from atomic coordinates, J. Appl. Cryst, vol.28, pp.768-773, 1995.

R. S. Svergun-d, . H. Koch-m, . Sayers-z, S. Kuprin, and . Zaccai-g, , 1998.

, Protein hydration in solution: experimental observation by X-ray and neutron scattering, Proc. Natl. Acad. Sci, vol.95, pp.2267-2272

V. Talanquer and . W. Oxtoby-d, Crystal nucleation in the presence of a metastable critical point, J. Chem. Phys, vol.109, issue.1, pp.223-227, 1998.

. Tanaka-s, M. Ataka, K. Onuma, J. P. Astier, and . Veesler-s, PH-dependent oligomerisation of BPTI in undersaturated ans supersaturated solutions studied by DLS, J. Crystal Growth, vol.237, pp.289-294, 2002.

. Tanford, Physical Chemistry of Macromolecules, 1961.

. G. Taratuta-v, . Holsbach-a, . M. Thurston-g, D. Blankstein, and . B. Benedek-g, Liquid-Liquid phase separation of aqueous lysozyme solutions: Effect of pH and salt identity, J. Phys. Chem, vol.94, pp.2140-2144, 1990.

. Tardieu-a, Thermodynamics and structure -concentrated solutions -structured disorder in vision, pp.146-154, 1994.

. Tardieu-a, V. A. Le, M. Malfois, F. Bonneté, S. Finet et al., Proteins in solution : from X-ray scattering intensities to interaction potentials, J. Cryst . Growth, vol.196, pp.196-203, 1999.

. Tardieu-a, F. Veretout, B. Krop, and . Slingsby-c, Protein interactions in the calf eye lens : interactions between b-crystallins are repulsive whereas in g-crystallins they are attractive, Eur. Biophys. J, vol.21, pp.1-12, 1992.

J. A. Thomson, P. Schurtenberger, . M. Thurston-g, and . B. Benedek-g, , 1987.

, Binary liquid phase separation and critical phenomena in a protein/water solution, Proc. Natl. Acad. Sci, vol.84, pp.7079-7083

A. N. Timasheff-s, Mechanism of protein precipitation and stabilisation by co-solvents, J. Cryst. Growth, vol.90, pp.39-46, 1988.

. Tuchsen-e, Properties of the protein solvent interface, studied by deuterium exchange in basic pancreatic trypsin inhibitor, Protides Biol. Fluids, vol.35, pp.437-448, 1987.

. Vachette-p, Small angle X-Ray scattering by solutions of biological macromolecules. International school of physics, Enrico Fermi" Course CXXVIII, 1996.

. Veesler-s, N. Ferte, M. S. Costes, M. Czjzek, and A. P. , Temperature and pH effect on the polymorphism of aprotinin (BPTI) in sodium bromide solutions, Crystal growth & design, vol.4, issue.6, pp.1137-1141, 2004.

. Veesler-s, S. Lafont, S. Marcq, J. P. Astier, and . Boistelle-r, Prenucleation, crystal growth and polymorphism of some proteins, J. Cryst. Growth, vol.168, pp.124-129, 1996.

P. G. Vekilov, Dense liquid precursor for the nucleation of ordered solid phases from solution, Crystal growth & design, vol.4, pp.671-685, 2004.

. G. Vekilov-p, M. Ataka, and . Katsura-t, Growth processes of protein crystals revealed by laser michelson interferometry investigation, Acta Cryst. D, vol.51, pp.207-219, 1995.

W. T. Vetterling, Numerical recipes -example book (Fortran), 1986.

. Vidal-o, Cristallogenèse d'un lysozyme en gel d'agarose et en gel de silice. Sciences de la matière, 1997.

. A. Vitale-s and . L. Katz-j, Liquid droplet dispersions formed by homogeneous liquidliquid nucleation: "the Ouzo effect, Langmuir, vol.19, pp.4105-4110, 2003.

. Vivares-d, Interactions en solution et cristallisation de l'urate oxydase, 2003.

. Vivares-d, L. Belloni, A. Tardieu, and . Bonnete-f, Catching the PEG-induced attractive interaction between proteins, Eur. Phys. J. E, vol.9, pp.15-25, 2002.

D. Vivares and . Bonnete-f, X-Ray scattering studies of Aspergillus flavus urate oxidase: towards a better understanding of PEG effects on the crystallization of large proteins, Acta Cryst. D, vol.58, pp.472-479, 2002.

D. Vivares and . Bonnete-f, Liquid-liquid phase separation in urate oxidase/PEG mixtures: characterization and implications for protein crystallization, J. Phys. Chem. B, vol.108, pp.6498-6507, 2004.

. Vivares-d, . W. Kaler-e, and . M. Lenhoff-a, Quantitative imaging by confocal scanning fluorescence microscopy of protein crystallization via liquid-liquid phase separation, Acta Cryst. D, vol.61, pp.819-825, 2005.

W. J. Huber-r, Pancreatic Tripsin Inhibitor, J. Mol. Biol, vol.167, pp.911-917, 1983.

. R. Wills-p and . Georgalis-y, Concentration dependence of the diffusion coefficient of a dimerizing protein: BPTI, J. Phys. Chem, vol.85, pp.3978-3984, 1981.

. Wlodawer-a, J. Deisenhofer, and . Huber-r, Comparison of two higly refined structures of BPTI, J. Mol. Biol, vol.193, pp.145-156, 1987.

. Wlodawer-a, J. Nachman, . L. Gilliland-g, W. Gallagher, and . Woodward-c, Structure of form III crystals of BPTI, J. Mol. Biol, vol.198, pp.469-480, 1987.

. R. Wolde-p and . Frenkel-d, Enhancement of protein crystal nucleation by critical density fluctuations, Science, vol.277, 1997.

. Ye-x, . Narayanan-t, . Tong-p, J. S. Huang, . Y. Lin-m et al.,

L. J. , Depletion interactions in colloid-polymer mixtures, Phys. Rev. E, vol.54, issue.6, pp.6500-6510, 1996.

P. Zielenkiewicz, Y. Georgalis, and . Saenger-w, Self-association of BPTI: specific or nonspecific?, Biopolymers, vol.31, pp.1347-1349, 1991.

. Bibliographie-________________________________________________________________________________,