Etude du phénomène d'adhésion chez la larve d'huître creuse Crassostrea gigas au stade pédivéligère

Abstract : Oysters show a two-phase life cycle: pelagic larvae adhere before metamorphosis into benthic life. Larval adhesion occurs at the pediveliger stage by secretion of a bioadhesive produced by a specialized organ: the foot. The oyster Crassostrea gigas is an organism of economic and ecological importance, and a model for study in marine biology, but the phenomenon of adhesion in the pediveliger larvae is poorly documented. A morphological description of the pediveliger larvae by histology and electron microscopy was performed to describe the glands responsible for the secretion of the adhesive.A predominantly proteinaceous composition of the adhesive was revealed by histochemistry and FTIR spectroscopy. An in silico analysis of available transcriptomic data from C. gigas was made to identify genes probably involved in adhesion. Two proteomic analyses, performed on whole larvae and on the secreted adhesive, characterizing proteins related to biosynthesis and adhesive structure. A collagen-like protein appears to be involved in the adhesive structure of C. gigas. This first approach to the study of the adhesion of C. gigas makes it possible to consider the biotechnological enhancement of the identified molecules. Despite their toxicity, synthetic adhesives dominate the world market. The development of biomimetic adhesives, based on marine bioadhesive strategies could be an alternative, and allowing furthermore bonding in wet condition.
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Valentin Foulon. Etude du phénomène d'adhésion chez la larve d'huître creuse Crassostrea gigas au stade pédivéligère. Autre [q-bio.OT]. Université de Bretagne occidentale - Brest, 2018. Français. ⟨NNT : 2018BRES0094⟩. ⟨tel-02130552v2⟩

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