Acousto-fluidique à ondes évanescentes, application à l'organisation de cultures de cellules adhérentes

Abstract : It has been shown that the use of acoustic waves enables nanoparticles, microbubbles, drops or microbeads, living cells and fluids to be moved, sorted, or mixed in a contactless and label-free manner. Here, we take advantage of the acoustic radiation force to manipulate living cells. Most of the applications and their associated techniques rely on the use of the so-called SAW (Rayleigh Surface Acoustic waves). This technique is powerful but requires piezoelectric substrates and suffers from a high damping due to radiation losses in the supersonic regime. Here, we work instead in the subsonic regime of propagation which allows us to generate an evanescent field ("Scholte" waves) thanks to a thin substrate. This wave presents very interesting characteristics since acoustic energy is concentrated in the vicinity of the substrate where objects are located. Moreover, the propagation is lossless and doesn't require any substrate or particular medium. We then showed the potential of this new approach through key-applications in microfluidics. This device enables to establish patterns and to concentrate cells in a flow. We have also designed a rotating acoustic field and shown the possibility of trapping and spinning of individual cells. We also describe a blood plasma characterization method by acoustic "centrifugation" within a drop. In a second part, we have designed a network of switchable acoustic traps compatible with living cells in order to study its effect on a population of adherent cells in culture. It reveals a change of cells behaviour depending on the phenotype. This work opens the way to the use of acoustics in the study of mechanotransductive effects on cells population.
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Vivian Aubert. Acousto-fluidique à ondes évanescentes, application à l'organisation de cultures de cellules adhérentes. Acoustique [physics.class-ph]. Université Pierre et Marie Curie - Paris VI, 2017. Français. ⟨NNT : 2017PA066367⟩. ⟨tel-01817015⟩

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