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, -hydroxy-2-methoxybenzylidene)-4-oxo-2-thioxothiazolidin-3-yl)acetic acid (HBRAA-2OM) Orange powder (74 %), p.1068

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1. Hz, 87 (s, 3H); 13 C NMR (75 MHz, CD 3 SOCD 3 , ? in ppm): 193, p.71

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, -hydroxy-2, 5-dimethylbenzylidene)-4-oxo-2-thioxothiazolidin-3-yl) acetic acid (HBRAA-2,5DM) Orange powder (59 %)

, 17 (s, 1H), 6.77 (s, 1H), 4.72 (s, 2H), 2.36 (s, 3H), 2.15 (s, 3H); 13 C NMR (75 MHz, CD 3 SOCD 3 , ? in ppm): 1936; MS (ESI): m/z 322, pp.36-3220214

J. =. , -thioxothiazolidin-3-yl)acetic acid (HBRAA-3E) Yellow powder (62 %) 1 H NMR (300 MHz, CD 3 SOCD 3 , ? in ppm): 13.44 (s, 1H), 10.49 (s, 1H), 7.77 (s, 1H), 7.41 (s, 1H), ), 2.59 (q, J = 7.2 Hz, 2H), 1.17 (t, pp.322-322

H. Esi, , p.212

, -thioxothiazolidin-3-yl)acetic acid (HBRAA-3OE) Yellow powder (59 %) 1 H NMR (300 MHz, CD 3 SOCD 3 , ? in ppm): 13.43 (s, Yeast cell sorting Yeast library (about 1 × 10 9 cells) was grown overnight (30°C, 280 rpm) in 1 L of SD (20 g/L dextrose, 6.7 g/L yeast nitrogen base, 1.92 g/L yeast synthetic dropout without tryptophane Yeast culture was diluted to OD 600 1 in 1L of SD and grown (30°C, 280 rpm) until OD 600 2-5. 5 × 10 9 cells yeast cells were then collected and grown for 36 h (23°C, pp.280-281

, 5× 10 8 induced cells were then pelleted by centrigugation (25°C, 3 min 1 g/L bovine serum albumin, pH 7.4), and incubated for 30 min at room temperature in 200 ?L of 1/250 primary antibody chicken antic-Myc IgY (Life Technologies) solution in DPBS-BSA. Cells were then washed with 10 mL DPBS-BSA, and incubated in 200 ?L of 1/100 secondary antibody Alexa Fluor® 647?goat anti-rabbit IgG, Na 2 HPO 4 -7H 2 O, 1% penicillin-streptomycin 10 washed with 10 mL DPBS-BSA (137 mM NaCl, 2.7 mM KClLife Technologies) solution in DPBS-BSA for 20 min on ice. After washing with DPBS, cells were incubated in 10 mL DPBS supplemented with

, Beckman Coulter) equipped with a 488 nm and a 640 nm laser. The sorted cells were collected in SD, grown overnight (30°C, 240 rpm) and spread on SD plates, MoFlo? Astrios Cell Sorter

, Plates were incubated for 60 h at 30°C. The cell lawn was collected in SD supplemented with 30% glycerol, aliquoted and frozen or directly used in the next round

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