Realization and study of substrates with modular rigidity and integratable devices for cellular and tissue engineering

Abstract : The purpose of this work is to develop manufacturable cell culture substrates and devices for large scale applications. By using both conventional and non-conventional lithography techniques, we firstly fabricated dense elastomer pillar arrays with height gradient for cell migration studies and we observed remarkable cell elongation and directed cell migration, all depending on the strength of the stiffness gradient. Elastomer micropillars could also be organized in ripple-like height gradient patterns, showing similar cell behaviors. Based on a biomimetic approach, we produced nanofibers on both side of a membrane with through holes for three-dimensional cell adhesion and migration. Our results showed that such a 3D scaffold can promote the cell infiltration and proliferation. Finally, we used micropillar arrays of different height as stiffness controlled substrate for cardiomyocytes differentiation from human induced pluripotent stem cells (hiPSCs). With the help of an elastomer stencil, uniform embryoids could be obtained and derived to the targeting cells on the substrate of different stiffness, showing a clear stiffness dependence of the substrates.
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Bin Wang. Realization and study of substrates with modular rigidity and integratable devices for cellular and tissue engineering. Analytical chemistry. PSL Research University, 2017. English. ⟨NNT : 2017PSLEE043⟩. ⟨tel-01816037⟩

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