Régulations traductionnelles de l'embryon précoce d'oursin : recrutement des ARNm dans les polysomes à la fécondation

Abstract : Protein synthesis is a crucial step for gene expression regulation. In many animal species, the early steps of development are based on translation of stored maternal mRNAs. Sea urchin embryo is a powerful model to study translational control during early development. Fertilization triggers the activation of translational machinery, leading to the increase of protein synthesis which is necessary to cell cycle entry and early embryonic development. Translational machinery modifications are responsible for the polysomal recruitment of the stored maternal mRNAs. Thus, are all the stored maternal mRNAs translated, or is there any selection of the translated mRNAs? If so, what are the mechanisms driving this selectivity? Over this work, we obtained the entire subset of the translationally regulated mRNAs, and demonstrated that only a part of the stored maternal mRNAs is actively translated at sea urchin fertilization, with an important enrichment of mRNAs coding for regulatory proteins. Finally, this work highlighted the diversity and the complexity of the signaling network upstream the selective polysomal recruitment.
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Héloïse Chassé. Régulations traductionnelles de l'embryon précoce d'oursin : recrutement des ARNm dans les polysomes à la fécondation. Biologie du développement. Université Pierre et Marie Curie - Paris VI, 2015. Français. ⟨NNT : 2015PA066503⟩. ⟨tel-01330117⟩

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