Faster and less phototoxic 3D fluorescence microscopy using a versatile compressed sensing scheme
Résumé
Three-dimensional fluorescence microscopy based on Nyquist sampling of focal planes faces harsh trade-offs between acquisition time, light exposure, and signal-to-noise. We propose a 3D compressed sensing approach that uses temporal modulation of the excitation intensity during axial stage sweeping and can be adapted to fluorescence microscopes without hardware modification. We describe implementations on a lattice light sheet microscope and an epifluorescence microscope, and show that images of beads and biological samples can be reconstructed with a 5-10 fold reduction of light exposure and acquisition time. Our scheme opens a new door towards faster and less damaging 3D fluorescence microscopy., "Metamaterial apertures for computational imaging," Science 310, 399 (2013). 12. J. Shin, B. T. Bosworth, and M. A. Foster, "Compressive fluorescence imaging using a multi-core fiber and spatially dependent scattering," Opt Lett 109, 42 (2017). 13. L. Gao, J. Liang, C. Li, and L. V. Wang, "Single-shot compressed ultrafast photography at one hundred billion frames per second," Nature 516, 74-77 (2014). 14. J. Liang, C. Ma, L. Zhu, Y. Chen, L. Gao, and L. V. Wang, "Single-shot real-time video recording of a photonic Mach cone induced by a scattered light pulse," Sci Adv 3, e1601814 (2017). 15. E. McLeod and A. Ozcan, "Unconventional methods of imaging: computational microscopy and compact implementations ,"
Domaines
Santé publique et épidémiologie
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