A method for selecting modified DNAzymes without the use of modified DNA as a template in PCR

Marleen Renders (1) , Emily Miller (1) , Marcel Hollenstein (1) , David Perrin (1)

1 UBC - University of British Columbia

Modified DNAzyme selections typically depend on recopying catalytically active modified DNA (mDNA) into cDNA in a PCR amplification step. However mDNA is often a poor template in PCR. Herein we propose a selection method in which the catalytically active, mDNA strand is covalently linked to the unmodified DNA template strand from which it was polymerized. Following selection, the unmodified DNA template is amplified in a PCR instead of the mDNA. This method circumvents the PCR amplification of mDNA.

Domaines: Chimie; Chimie organique

Marleen Renders, Emily Miller, Marcel Hollenstein, David Perrin. A method for selecting modified DNAzymes without the use of modified DNA as a template in PCR. Chemical Communications, 2015, 51 (7), pp.1360-1362. ⟨10.1039/c4cc07588a⟩. ⟨pasteur-02012270⟩