Early over expression of messenger RNA for multiple genes, including insulin, in the Pancreatic Lymph Nodes of NOD mice is associated with Islet Autoimmunity

Béatrice Regnault (1) , José Osorio y Fortéa (2) , Dongmei Miao (3) , George S Eisenbarth (3) , Evie Melanitou (2)

1 Génopole
2 Immunophysiologie et Parasitisme Intracellulaire
3 BDC - Barbara Davis Center for Childhood Diabetes

Background: Autoimmune diabetes (T1D) onset is preceded by a long inflammatory process directed against the insulin-secreting β cells of the pancreas. Deciphering the early autoimmune mechanisms represents a challenge due to the absence of clinical signs at early disease stages. The aim of this study was to identify genes implicated in the early steps of the autoimmune process, prior to inflammation, in T1D. We have previously established that insulin autoantibodies (E-IAA) predict early diabetes onset delineating an early phenotypic check point (window 1) in disease pathogenesis. We used this sub-phenotype and applied differential gene expression analysis in the pancreatic lymph nodes (PLN) of 5 weeks old Non Obese Diabetic (NOD) mice differing solely upon the presence or absence of E-IAA. Analysis of gene expression profiles has the potential to provide a global understanding of the disease and to generate novel hypothesis concerning the initiation of the autoimmune process.

Domaines: Génétique animale; Génétique humaine; Endocrinologie et métabolisme; Maladies infectieuses; Organisation et fonctions cellulaires [q-bio.SC]

Béatrice Regnault, José Osorio y Fortéa, Dongmei Miao, George S Eisenbarth, Evie Melanitou. Early over expression of messenger RNA for multiple genes, including insulin, in the Pancreatic Lymph Nodes of NOD mice is associated with Islet Autoimmunity. BMC Medical Genomics, 2009, 2 (63), ⟨10.1186/1755-8794-2-63⟩. ⟨pasteur-01643691⟩