An optimized protocol for isolating lymphoid stromal cells from the intestinal lamina propria

Igor Stzepourginski (1) , Gérard Eberl (1) , Lucie Peduto (1)

1 Développement des Tissus Lymphoïdes

Mesenchymal stromal cells in lymphoid organs, also called lymphoid stromal cells (LSCs), play a pivotal role in immunity by forming specialized microenvironments that provide signals for leukocyte migration, positioning, and survival. Best characterized in lymphoid organs, LSCs are also abundant in the intestinal mucosa, which harbors a rich repertoire of immune cells. However, the lack of efficient procedures for isolation and purification of LSCs from the intestine has been a major limitation to their characterization. Here we report a new method to efficiently isolate, in addition to immune cells, viable lymphoid stromal cells and other stromal subsets from the intestinal lamina propria for subsequent phenotypic and functional analysis.

Mots clés: Flow cytometry; Intestinal lymphoid stromal cells; Mucosal immunity; Stromal cell viability/yield; Tissue dissociation

Domaines: Immunologie; Hépatologie et Gastroentérologie; Biologie cellulaire

Igor Stzepourginski, Gérard Eberl, Lucie Peduto. An optimized protocol for isolating lymphoid stromal cells from the intestinal lamina propria. Journal of Immunological Methods, 2015, 421, pp.14 - 19. ⟨10.1016/j.jim.2014.11.013⟩. ⟨pasteur-01402711⟩