Characterization of glucuronic acid containing glycolipid in Aspergillus fumigatus mycelium.

Thierry Fontaine (1) , Claude Lamarre (2) , Catherine Simenel (3) , Karine Lambou (1) , Bernadette Coddeville (4) , Muriel Delepierre (3) , Jean-Paul Latgé (1)

1 Aspergillus
2 CHUQ - Centre hospitalier universitaire de Québec
3 Résonance Magnétique Nucléaire des Biomolécules
4 UGSF - Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576

A glucuronic acid containing glycerolipid was isolated from the filamentous fungi Aspergillus fumigatus. This acidic glycolipid was extracted from the membrane of mycelium and purified by two successive chromatographic steps on DEAE-Sephadex and Silica columns. Chemical structural analysis was performed using methylation, gas-chromatography, gas-chromatography-mass spectrometry, nano-electrospray mass spectrometry and (1)H/(13)C NMR spectra. The corresponding structure is a 3-(O-alpha-glucuronyl)-1,2-diacyl-sn-glycerol, where acyl chains are mainly C(16:0), C(18:0), C(18:1), and C(18:2). This alpha-GlcA-diacylglycerol is not present in fungal conidia. This acidic glycerolipid is described here for the first time in a fungal species. Two homologs of UDP-glucose dehydrogenase that convert UDP-glucose into UDP-glucuronic acid, are present in A. fumigatus genome, UGD1 and UGD2. Gene deletion showed that only UGD1 is essential for the biosynthesis of GlcA-DG. However, no particular phenotype has been observed in the Ugd1Delta mutant. Biological function of this acidic glycolipid remains unknown in A. fumigatus.

Domaines: Biochimie, Biologie Moléculaire

Thierry Fontaine, Claude Lamarre, Catherine Simenel, Karine Lambou, Bernadette Coddeville, et al.. Characterization of glucuronic acid containing glycolipid in Aspergillus fumigatus mycelium.. Carbohydrate Research, 2009, 344 (15), pp.1960-7. ⟨10.1016/j.carres.2009.07.012⟩. ⟨pasteur-00512059⟩