Genetic analysis of Thailand hantavirus in Bandicota indica trapped in Thailand

Jean-Pierre Hugot (1, 2) , Angelina Plyusnina (3) , Vincent Herbreteau (2) , Kirill Nemirov (4) , Juha Laakkonen (5) , Ake Lundkvist (4) , Yupin Suputtamongkol (6) , Heikki Henttonen (5) , Alexander Plyusnin (3, 4)

1 OSEB - Origine, structure et évolution de la biodiversité
2 IRD UR178
3 Department of Virology [Helsinki]
4 Swedish Institute for Infectious Disease Control
5 Finnish Forest Research Institute, Vantaa Research Unit
6 Siriraj Hospital, Mahidol University

Sixty one tissue samples from several rodent species trapped in five provinces of Thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. Four samples, all from the great bandicoot rat Bandicota indica, were confirmed positive for the hantaviral N-antigen. Two of them were trapped in Nakhon Pathom province, the other two in Nakhon Ratchasima province, approximately 250 km from the other trapping site. When analysed by RT-nested PCR, all four rodents were found positive for the hantaviral S- and M-segment nucleotide sequences. Genetic analysis revealed that the four newly described wild-type strains belong to Thailand hantavirus. On the phylogenetic trees they formed a well-supported cluster within the group of Murinae-associated hantaviruses and shared a recent common ancestor with Seoul virus.

Mots clés: VIRUS; RAT; IMMUNOLOGIE; ENZYME; PHYLOGENIE; ETUDE COMPARATIVE; ANALYSE GENETIQUE; TECHNIQUE RT PCR; THAILANDE; NAKHON RATCHASIMA; SAKHON NAKHON; PHRAE

Domaines: Santé

Jean-Pierre Hugot, Angelina Plyusnina, Vincent Herbreteau, Kirill Nemirov, Juha Laakkonen, et al.. Genetic analysis of Thailand hantavirus in Bandicota indica trapped in Thailand. Virology Journal, 2006, 3, pp.NIL_1-NIL_9. ⟨10.1186/1743-422X-3-72⟩. ⟨ird-00380483⟩