The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020

J. F. Huggett (1) , A. S. Whale , W. De Spiegelaere , W. Trypsteen , A. A. Nour , Y.-K. Bae , V. Benes , D. G. Burke , Philippe Corbisier , Megan Cleveland , Alison S Devonshire , Lianhua Dong , Deniela Drandi , Carole Foy , J. A. Garson , J. Hellemans , H.-J. He , M. Kubista , A. Lievens , M. Makrigiorgos , M. Milavec , R. D. Mueller , T. Nolan , D. O'Sullivan , M. W. Pfaffl , S. Roediger , E. Romsos , G. Shipley , V. Taly (2) , A. Untergasser , C. Wittwer , S. Bustin , And J. Vandesompele.

1 UNIS - University of Surrey
2 CRC (UMR_S_1138 / U1138) - Centre de Recherche des Cordeliers

Digital PCR (dPCR) has developed considerably since the publication of the Minimum Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with advances in instrumentation, software, applications, and our understanding of its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult and preanalytical steps required to purify, concentrate, and modify nucleic acids can lead to measurement error. To assist independent corroboration of conclusions, comprehensive disclosure of all relevant experimental details is required. To support the community and reflect the growing use of dPCR, we present an update to dMIQE, dMIQE2020, including a simplified dMIQE table format to assist researchers in providing key experimental information and understanding of the associated experimental process. Adoption of dMIQE2020 by the scientific community will assist in standardizing experimental protocols, maximize efficient utilization of resources, and further enhance the impact of this powerful technology.

Mots clés: MIQE; dMIQE; dPCR; digital PCR.

Domaines: Sciences du Vivant [q-bio]

J. F. Huggett, A. S. Whale, W. De Spiegelaere, W. Trypsteen, A. A. Nour, et al.. The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020. Clinical Chemistry, 2020, 66 (8), pp.1012-1029. ⟨10.1093/clinchem/hvaa125⟩. ⟨inserm-02992959⟩