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RNA polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses

Abstract : Rift Valley fever virus (RVFV, Bunyaviridae, Phlebovirus) is a mosquito-transmitted arbovirus that causes human and animal diseases in sub-Saharan Africa and was introduced into the Arabian Peninsula in 2000. Here, we describe a method of reverse genetics to recover infectious RVFV from transfected plasmids based on the use of the cellular RNA polymerase I promoter to synthesize viral transcripts. We compared its efficiency with a system using T7 RNA polymerase and found that both are equally efficient for the rescue of RVFV generating titers of approx. 10(7) to 10(8) pfu/ml. We used the RNA polymerase I-based system to rescue both attenuated MP12 and virulent ZH548 strains as well as chimeric MP12-ZH548 viruses, and in addition RVFV expressing reporter proteins.
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https://www.hal.inserm.fr/inserm-02461398
Contributor : Nicolas Le May Connect in order to contact the contributor
Submitted on : Thursday, January 30, 2020 - 4:18:17 PM
Last modification on : Thursday, April 7, 2022 - 10:10:26 AM

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Agnès Billecocq, Nicolas Gauliard, Nicolas Le May, Richard Elliott, Ramon Flick, et al.. RNA polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses. Virology, Elsevier, 2008, 378 (2), pp.377-384. ⟨10.1016/j.virol.2008.05.033⟩. ⟨inserm-02461398⟩

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