Specific IgE and IgG measured by the MeDALL allergen-chip depend on allergen and route of exposure: The EGEA study

Valérie Siroux (1) , Christian Lupinek (2) , Yvonne Resch (2) , Mirela Curin (2) , Jocelyne Just (3, 4) , Thomas Keil (5, 6) , Renata Kiss (2) , Karin Lodrup Carlsen (7, 8) , Erik Melen (9, 10) , Rachel Nadif (11) , Isabelle Pin (1) , Ingebjørg Skrindo (8, 12) , Susanne Vrtala (2, 13) , Magnus Wickman (10, 11) , Josep Maria (14, 15, 16, 17) , Rudolf Valenta (2) , Jean Bousquet (11, 18, 19)

1 IAB - Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble)
2 Division of Immunopathology - Department of Pathophysiology and Allergy Research [Vienna, Austria]
3 CHU Trousseau [APHP]
4 UPMC - Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie
5 Institute of Social Medicine - Epidemiology and Health Economics [Berlin, Germany]
6 Institute for Clinical Epidemiology and Biometry [Würzburg, Germany]
7 Department of Paediatrics [Oslo, Norway]
8 Institute of Clinical Medicine [Oslo]
9 Institute of Environmental Medicine [Stockholm, Sweden]
10 Sachs’ Children and Youth Hospital [Stockholm, Sweden]
11 VIMA - Vieillissement et Maladies chroniques : approches épidémiologique et de santé publique
12 Department of Otorhinolaryngology [Lørenskog, Norway]
13 Department of Pathophysiology & Allergy Research - Christian Doppler Laboratory for the Development of Allergen chips [Vienna, Austria]
14 CREAL - Center for Research in Environmental Epidemiology
15 IMIM - Hospital del Mar Medical Research Institute [Barcelona, Spain]
16 UPF - Universitat Pompeu Fabra [Barcelona]
17 CIBERESP - Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública = Consortium for Biomedical Research of Epidemiology and Public Health
18 MeDALL-FP7 - Mechanisms of the Development of Allergy
19 CHU Montpellier

BACKGROUND: The nature of allergens and route and dose of exposure may affect the natural development of IgE and IgG responses. OBJECTIVE: We sought to investigate the natural IgE and IgG responses toward a large panel of respiratory and food allergens in subjects exposed to different respiratory allergen loads. METHODS: A cross-sectional analysis was conducted in 340 adults of the EGEA (Epidemiological study of the Genetics and Environment of Asthma, bronchial hyperresponsiveness and atopy) (170 with and 170 without asthma) cohort. IgE and IgG responses to 47 inhalant and food allergen components were analyzed in sera using allergen microarray and compared between 5 French regions according to the route of allergen exposure (inhaled vs food allergens). RESULTS: Overall 48.8% of the population had allergen-specific IgE levels of 0.3 ISAC standardized units (ISU) or more to at least 1 of the 47 allergens with no significant differences across the regions. For ubiquitous respiratory allergens (ie, grass, olive/ash pollen, house dust mites), specific IgE did not show marked differences between regions and specific IgG (≥0.5 ISU) was present in most subjects everywhere. For regionally occurring pollen allergens (ragweed, birch, cypress), IgE sensitization was significantly associated with regional pollen exposure. For airborne allergens cross-reacting with food allergens, frequent IgG recognition was observed even in regions with low allergen prevalence (Bet v 1) or for allergens less frequently recognized by IgE (profilins). CONCLUSIONS: The variability in allergen-specific IgE and IgG frequencies depends on exposure, route of exposure, and overall immunogenicity of the allergen. Allergen contact by the oral route might preferentially induce IgG responses.

Mots clés: allergen components; cohort; microarray; respiratory allergens; epidemiology; food allergens; EGEA; IgE; IgG; MeDALL

Domaines: Santé publique et épidémiologie; Sciences du Vivant [q-bio]

Valérie Siroux, Christian Lupinek, Yvonne Resch, Mirela Curin, Jocelyne Just, et al.. Specific IgE and IgG measured by the MeDALL allergen-chip depend on allergen and route of exposure: The EGEA study. Journal of Allergy and Clinical Immunology, 2017, 139 (2), pp.643 - 654. ⟨10.1016/j.jaci.2016.05.023⟩. ⟨inserm-01815134⟩