Syndecan-1 regulates the biological activities of interleukin-34 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue BBA - Biochimica et Biophysica Acta Année : 2015

Syndecan-1 regulates the biological activities of interleukin-34

Résumé

IL-34 is a challenging cytokine sharing functional similarities with M-CSF through M-CSFR activation. It also plays a singular role that has recently been explained in the brain, through a binding to the receptor protein tyrosine phosphatase RPTPβ/ζ. The aim of this paper was to look for alternative binding of IL-34 on other cell types. Myeloid cells (HL-60, U-937, THP-1) were used as cells intrinsically expressing M-CSFR, and M-CSFR was expressed in TF-1 and HEK293 cells. IL-34 binding was studied by Scatchard and binding inhibition assays, using 125I-radiolabelled cytokines, and surface plasmon resonance. M-CSFR activation was analysed by Western blot after glycosaminoglycans abrasion, syndecan-1 overexpression or repression and addition of a blocking anti-syndecan antibody. M-CSF and IL-34 induced different patterns of M-CSFR phosphorylations, suggesting the existence of alternative binding for IL-34. Binding experiments and chondroitinase treatment confirmed low affinity binding to chondroitin sulphate chains on cells lacking both M-CSFR and RPTPβ/ζ. Amongst the proteoglycans with chondroitin sulphate chains, syndecan-1 was able to modulate the IL-34-induced M-CSFR signalling pathways. Interestingly, IL-34 induced the migration of syndecan-1 expressing cells. Indeed, IL-34 significantly increased the migration of THP-1 and M2a macrophages that was inhibited by addition of a blocking anti-syndecan-1 antibody. This paper provides evidence of alternative binding of IL-34 to chondroitin sulphates and syndecan-1 at the cell surface that modulates M-CSFR activation. In addition, IL-34-induced myeloid cell migration is a syndecan-1 dependent mechanism.
Fichier principal
Vignette du fichier
Segaliny et al BBA Mol Cel Res 2015 (text UN marked).pdf (1.97 Mo) Télécharger le fichier
SupplementaryDataBBA.pdf (794.32 Ko) Télécharger le fichier
Loading...

Dates et versions

inserm-01644783 , version 1 (22-11-2017)

Identifiants

Citer

Aude Segaliny, Régis Brion, Erwan Mortier, Mike Maillasson, Michel Chérel, et al.. Syndecan-1 regulates the biological activities of interleukin-34: Syndecan-1 is a co-receptor for IL-34. BBA - Biochimica et Biophysica Acta, 2015, 1853 (5), pp.1010 - 1021. ⟨10.1016/j.bbamcr.2015.01.023⟩. ⟨inserm-01644783⟩
77 Consultations
117 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More