 |
Journal articles
Interleukin 34 expression is associated with synovitis severity in rheumatoid arthritis patients.
Abstract : OBJECTIVES: Interleukin (IL) 34 is a new cytokine implicated in macrophage differentiation and osteoclastogenesis. This study assessed IL-34 expression in the tissue of patients with rheumatoid arthritis (RA). METHODS: Immunohistochemistry was performed in synovial biopsies from patients with RA (n=20), osteoarthritis (n=3) or other inflammatory arthritis (n=4). IL-34 was detected in the synovial fluid by ELISA and its messenger RNA expression was studied by quantitative PCR in rheumatoid synovial fibroblasts after stimulation by tumour necrosis factor α (TNFα) and IL-1β. Wild-type, jnk1(-/-)-jnk2(-/-) and nemo(-/-) murine fibroblasts and pharmacological inhibition were used to determine the involvement of nuclear factor kappa B (NF-κB) and JNK in that effect. RESULTS: IL-34 was expressed in 24/27 biopsies, with three samples from RA patients being negative. A significant association was found between IL-34 expression and synovitis severity. Levels of IL-34 and the total leucocyte count in synovial fluid were correlated. TNFα and IL-1β stimulated IL-34 expression by synovial fibroblasts in a dose/time-dependent manner through the NF-κB and JNK pathway. CONCLUSION: This work for the first time identifies IL-34 expression in the synovial tissue of patients with arthritis. This cytokine, as a downstream effector of TNFα and IL-1β, may contribute to inflammation and bone erosions in RA.
https://www.hal.inserm.fr/inserm-00667486
Contributor : D* Heymann <>
Submitted on : Tuesday, February 7, 2012 - 5:11:23 PM
Last modification on : Thursday, February 7, 2019 - 5:10:44 PM
Long-term archiving on: : Tuesday, May 8, 2012 - 2:35:14 AM
Contributor : D* Heymann <>
Submitted on : Tuesday, February 7, 2012 - 5:11:23 PM
Last modification on : Thursday, February 7, 2019 - 5:10:44 PM
Long-term archiving on: : Tuesday, May 8, 2012 - 2:35:14 AM
Files
inserm-00667486_edited.pdf
Files produced by the author(s)
- Chemel_et_al.pdf Files produced by the author(s)