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Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification.

Ewelina Kosior 1 Sylvain Bohic 2 Heikki Suhonen 1 Richard Ortega 3 Guillaume Devès 3 Asuncion Carmona 3 Florence Marchi 4 Jean Francois Guillet 5 Peter Cloetens 1, * 
* Corresponding author
2 INSERM U836, équipe 6, Rayonnement synchrotron et recherche médicale
ESRF - European Synchrotron Radiation Facility, GIN - Grenoble Institut des Neurosciences
4 CP
Physics department - UFR PHITEM, Surface Science Laboratory
Abstract : Hard X-ray fluorescence microscopy and magnified phase contrast imaging are combined to obtain quantitative maps of the projected metal concentration in whole cells. The experiments were performed on freeze dried cells at the nano-imaging station ID22NI of the European Synchrotron Radiation Facility (ESRF). X-ray fluorescence analysis gives the areal mass of most major, minor and trace elements; it is validated using a biological standard of known composition. Quantitative phase contrast imaging provides maps of the projected mass and is validated using calibration samples and through comparison with Atomic Force Microscopy and Scanning Transmission Ion Microscopy. Up to now, absolute quantification at the sub-cellular level was impossible using X-ray fluorescence microscopy but can be reached with the use of the proposed approach.
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Submitted on : Wednesday, January 25, 2012 - 11:48:33 AM
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Ewelina Kosior, Sylvain Bohic, Heikki Suhonen, Richard Ortega, Guillaume Devès, et al.. Combined use of hard X-ray phase contrast imaging and X-ray fluorescence microscopy for sub-cellular metal quantification.. Journal of Structural Biology, Elsevier, 2012, 177 (2), pp.239-47. ⟨10.1016/j.jsb.2011.12.005⟩. ⟨inserm-00662829⟩



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