A novel in vitro preparation: the intact hippocampal formation.

Ilgam Khalilov (1) , Monique Esclapez (1) , Igor Medina (1) , Djamila Aggoun (1) , Karri Lamsa (1) , Xavier Leinekugel (1) , Roustem Khazipov (1) , Yezekiel Ben-Ari (1)

1 Epilepsie et Ischemie Cerebrale

The intact hippocampal formation (IHF) of neonatal or young rats can be kept alive for an extended period in a fully submerged chamber with excellent morphological preservation. Field or patch-clamp recordings, intracellular Ca2+ measurements, and 3-D reconstruction of biocytin-filled neurons can be performed routinely. The generation and propagation of network-driven activities can be studied within the IHF or between connected intact structures such as the septum and the hippocampus or two hippocampi, and the use of a dual chamber enables the application of drugs separately to each structure. This preparation will be useful to study intact neuronal networks in the developing hippocampus in vitro.

Domaines: Biologie cellulaire

Ilgam Khalilov, Monique Esclapez, Igor Medina, Djamila Aggoun, Karri Lamsa, et al.. A novel in vitro preparation: the intact hippocampal formation.. Neuron, 1997, 19 (4), pp.743-9. ⟨inserm-00522462⟩