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Deregulation of Aurora kinase gene expression in human testicular germ cell tumours.

Abstract : The Aurora kinases regulate chromosome segregation and cytokinesis, and alterations in their expression associate with cell malignant transformation. In this study, we demonstrated by qRT-PCR analysis of 14 seminomas that Aurora-A mRNA was, with respect to control tissues, augmented in five of 14 tumour tissues by 2.17 +/- 0.30 fold (P < 0.05) and reduced in 9 to 0.38 +/- 0.10 (P < 0.01). Aurora-B mRNA was increased in 11 tumour tissues by 4.33 +/- 0.82 fold (P < 0.01) and reduced in 3 to 0.41 +/- 0.11 fold. Aurora-C mRNA was reduced to 0.20 +/- 0.32 fold (P < 0.01) in 13 seminomas and up-regulated in one case. Western blot experiments, performed on protein extracts of nine seminomas and six normal testes, showed an up-regulation of Aurora-B protein by 10.14 +/- 3.51 fold (P < 0.05), while Aurora-A protein was found increased in four seminomas by 2.16 +/- 0.43 (P < 0.05), unchanged in three and reduced in two tumour tissues. Aurora-C protein was increased by 9.2 +/- 2.90 fold (P < 0.05), suggesting that post-transcriptional mechanisms modulate its expression. In conclusion, we demonstrated that expression of Aurora kinases is deregulated in seminomas, suggesting that they may play a role in the progression of testicular cancers.
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Contributor : Hervé De Villemeur Connect in order to contact the contributor
Submitted on : Tuesday, August 24, 2010 - 11:07:17 AM
Last modification on : Wednesday, March 30, 2022 - 2:35:11 PM

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Enke Baldini, Yannick Arlot-Bonnemains, Marcella Mottolese, Steno Sentinelli, Barbara Antoniani, et al.. Deregulation of Aurora kinase gene expression in human testicular germ cell tumours.. Andrologia, Wiley, 2010, 42 (4), pp.260-7. ⟨10.1111/j.1439-0272.2009.00987.x⟩. ⟨inserm-00511226⟩



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