Skip to Main content Skip to Navigation
Journal articles

A purified population of multipotent cardiovascular progenitors derived from primate pluripotent stem cells engrafts in postmyocardial infarcted nonhuman primates.

Abstract : Cell therapy holds promise for tissue regeneration, including in individuals with advanced heart failure. However, treatment of heart disease with bone marrow cells and skeletal muscle progenitors has had only marginal positive benefits in clinical trials, perhaps because adult stem cells have limited plasticity. The identification, among human pluripotent stem cells, of early cardiovascular cell progenitors required for the development of the first cardiac lineage would shed light on human cardiogenesis and might pave the way for cell therapy for cardiac degenerative diseases. Here, we report the isolation of an early population of cardiovascular progenitors, characterized by expression of OCT4, stage-specific embryonic antigen 1 (SSEA-1), and mesoderm posterior 1 (MESP1), derived from human pluripotent stem cells treated with the cardiogenic morphogen BMP2. This progenitor population was multipotential and able to generate cardiomyocytes as well as smooth muscle and endothelial cells. When transplanted into the infarcted myocardium of immunosuppressed nonhuman primates, an SSEA-1+ progenitor population derived from Rhesus embryonic stem cells differentiated into ventricular myocytes and reconstituted 20% of the scar tissue. Notably, primates transplanted with an unpurified population of cardiac-committed cells, which included SSEA-1- cells, developed teratomas in the scar tissue, whereas those transplanted with purified SSEA-1+ cells did not. We therefore believe that the SSEA-1+ progenitors that we have described here have the potential to be used in cardiac regenerative medicine.
Complete list of metadatas

Cited literature [53 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00451770
Contributor : Michel Puceat <>
Submitted on : Monday, March 29, 2010 - 8:49:31 AM
Last modification on : Tuesday, June 30, 2020 - 2:45:17 PM
Document(s) archivé(s) le : Tuesday, September 28, 2010 - 12:07:50 PM

File

BlinNuryStefanovic_et_al_JCIac...
Files produced by the author(s)

Identifiers

Citation

Guillaume Blin, David Nury, Sonia Stefanovic, Tui Neri, Orianne Guillevic, et al.. A purified population of multipotent cardiovascular progenitors derived from primate pluripotent stem cells engrafts in postmyocardial infarcted nonhuman primates.. Journal of Clinical Investigation, American Society for Clinical Investigation, 2010, 120 (4), pp.1125-39. ⟨10.1172/JCI40120⟩. ⟨inserm-00451770⟩

Share

Metrics

Record views

1718

Files downloads

976